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Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

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Related Experiment Video

Updated: Jun 16, 2026

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Efficient Generation of Pancreas/Duodenum Homeobox Protein 1+ Posterior Foregut/Pancreatic Progenitors from hPSCs in Adhesion Cultures

Published on: March 27, 2019

Pancreatic Progenitor Commitment Is Marked by an Increase in Ink4a/Arf Expression.

Elena Montano¹, Alessandra Pollice¹, Valeria Lucci^1,2

¹Department of Biology, University of Naples "Federico II", 80147 Naples, Italy.

|August 27, 2021

Summary

Researchers identified Ink4a and Arf as markers for early pancreatic progenitor cells (PPCs) derived from mouse embryonic stem cells. This discovery aids understanding of pancreatic development and potential cell-based therapies.

Keywords:

ARF Cdkn2a INK4a Pancreatic Progenitor Cells embryonic stem cells

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Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors

Published on: March 7, 2017

Area of Science:

Developmental Biology
Stem Cell Biology
Molecular Medicine

Background:

Understanding early mammalian cell fate decisions is crucial for regenerative therapies.
Pancreatic Progenitor Cells (PPCs) are vital for pancreas maintenance and regeneration.
Lack of early endoderm markers hinders research into pancreatic cellular remodeling.

Purpose of the Study:

To identify novel molecular markers for early pancreatic progenitor cell specification.
To investigate the role of the Ink4a/Arf locus in pancreatic lineage commitment.
To explore epigenetic regulation of the Cdkn2a locus during pancreatic differentiation.

Main Methods:

Induced differentiation of mouse embryonic stem cells (mESCs) towards the pancreatic lineage.
Analysis of gene expression, specifically focusing on the Cdkn2a locus products (Ink4a and Arf).
Treatment with epigenetic drugs (epi-drugs) to assess chromatin remodeling's role.

Main Results:

Ink4a and Arf expression identified a naive pancreatic cellular state resembling PPC-like specification.
Epigenetic drug treatment suggested chromatin remodeling influences Cdkn2a locus regulation.
Established a link between Ink4a/Arf expression and early pancreatic progenitor identity.

Conclusions:

Ink4a and Arf serve as valuable markers for early pancreatic progenitor cells.
Chromatin remodeling plays a role in regulating the Cdkn2a locus during pancreatic differentiation.
Findings enhance understanding of pancreatic cellular ontogeny for stem cell-based therapies.