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Updated: Oct 22, 2025

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Simple Method for Establishing Primary Leporidae Skin Fibroblast Cultures.

Fábio A Abade Dos Santos1,2,3, C L Carvalho2, Isabel Almeida2

  • 1Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA), Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal.

Cells
|August 27, 2021
PubMed
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Researchers developed a rapid, cost-effective method for creating primary fibroblast cell cultures from leporid species. This technique overcomes limitations in existing immortalized cell lines, improving research accessibility for lagomorphs.

Area of Science:

  • Veterinary Medicine
  • Cell Biology
  • Animal Science

Background:

  • Limited availability of immortalized cell lines for diverse lagomorph species hinders research.
  • Primary cell cultures are essential for studies requiring species-specific models, including diagnostics.
  • Fibroblasts are robust cells suitable for various research applications, including viral isolation.

Purpose of the Study:

  • To establish a fast, inexpensive, and efficient method for generating primary fibroblast cell cultures from leporid species.
  • To provide a reliable in vitro cell culture system for lagomorph research and diagnostics.

Main Methods:

  • Utilized dispase II for dermal-epidermal separation of leporid tissues.
  • Employed subsequent enzymatic digestion with trypsin to isolate fibroblasts.
Keywords:
Leporidaedispase IImethodprimary cell cultureprimary fibroblastsvirus isolation

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  • Established primary fibroblast cell cultures from biopsies or embryos.

    • Main Results:

    • Achieved high viability yields and purity levels exceeding 85% for primary fibroblast cultures.
    • Successfully demonstrated the maintenance and potential immortalization of leporid fibroblastic cells.
    • Developed a cost-effective and rapid protocol for leporid cell culture establishment.

    Conclusions:

    • The described method offers a practical solution for creating primary leporid fibroblast cell cultures.
    • This advancement expands research capabilities in lagomorph biology, diagnostics, and disease modeling.
    • The protocol supports the long-term maintenance and potential immortalization of these valuable cell types.