Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.6K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intracrine FFA4 signaling controls lipolysis at lipid droplets.

Nature chemical biology·2025
Same author

Loss-of-function Gα<sub>s</sub> rare disease variants exert mutation-specific effects on GPCR signaling.

Science signaling·2025
Same author

Design, Synthesis, and Characterization of New δ Opioid Receptor-Selective Fluorescent Probes and Applications in Single-Molecule Microscopy of Wild-Type Receptors.

Journal of medicinal chemistry·2024
Same author

Revealing the tissue-level complexity of endogenous glucagon-like peptide-1 receptor expression and signaling.

Nature communications·2023
Same author

Filamin A organizes γ‑aminobutyric acid type B receptors at the plasma membrane.

Nature communications·2023
Same author

How Carvedilol activates β<sub>2</sub>-adrenoceptors.

Nature communications·2022

Related Experiment Video

Updated: Oct 22, 2025

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers
09:33

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

Published on: March 21, 2025

1.1K

Detecting Transient Trapping from a Single Trajectory: A Structural Approach.

Yann Lanoiselée1,2, Jak Grimes1,2, Zsombor Koszegi1,2

  • 1Institute of Metabolism and Systems Research, University of Birmingham, Birmingham B15 2TT, UK.

Entropy (Basel, Switzerland)
|August 27, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method to detect transient trapping events in single particle trajectories, revealing increased receptor trapping upon stimulation without altering free receptor diffusion. This advancement aids in understanding cellular dynamics.

Keywords:
confinementsingle particle trajectorystochastic processestrapping

More Related Videos

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

4.4K
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

5.2K

Related Experiment Videos

Last Updated: Oct 22, 2025

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers
09:33

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

Published on: March 21, 2025

1.1K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

4.4K
Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

5.2K

Area of Science:

  • Biophysics
  • Cell Biology
  • Physical Chemistry

Background:

  • Understanding particle dynamics is crucial for cellular processes.
  • Existing methods struggle to identify short-lived trapping events in single trajectories.
  • Intracellular and plasma membrane compartmentalization influence cellular functions.

Purpose of the Study:

  • To develop a new method for detecting transient trapping events in single particle trajectories.
  • To analyze changes in particle dynamics over time.
  • To investigate intracellular and plasma membrane compartmentalization.

Main Methods:

  • A novel method based on smoothed recurrence matrix measures.
  • Analysis considers both spatial and temporal trajectory structures.
  • Trajectory is investigated as a whole, avoiding sliding windows.

Main Results:

  • The method successfully detects transient trapping events.
  • It is applicable to short-lived trapping domains.
  • Application to beta-2 adrenergic receptors shows increased receptor trapping upon stimulation.
  • Free receptor diffusion remained unchanged.

Conclusions:

  • The new method enables explicit accounting of dynamic changes in single particle trajectories.
  • It provides valuable insights into intracellular and plasma membrane compartmentalization.
  • Receptor stimulation leads to enhanced trapping in specific cellular domains.