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Bombyx mori Nucleopolyhedrovirus p26 Is Associated with Viral Late Stage Replication

Jun-Qing Ge1, Zhu-Hong Wang2, Xi Chen1

  • 1Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Wusi Road 247, Fuzhou 350003, China.

Insects
|August 27, 2021
PubMed

Insights

The Bombyx mori nucleopolyhedrovirus p26 gene is an early viral gene involved in virus replication. Knockdown of p26 reduces budded virus production and affects late-stage viral gene expression.

Area of Science:

  • Virology
  • Molecular Biology
  • Insect Pathology

Background:

  • The Bombyx mori nucleopolyhedrovirus (BmNPV) p26 gene is conserved across Lepidoptera baculoviruses, suggesting functional importance.
  • Some baculoviruses possess multiple copies of the p26 gene, further highlighting its potential significance in the viral infection cycle.

Purpose of the Study:

  • To characterize the expression pattern and cellular localization of the BmNPV p26 gene.
  • To investigate the role of BmNPV p26 in viral replication and progeny production.

Main Methods:

  • Quantitative real-time PCR (Q-PCR) to analyze p26 transcript levels.
  • Western blot analysis to detect p26 protein expression.
  • Immunofluorescence microscopy to determine p26 cellular localization.
  • RNA interference (RNAi) to knockdown p26 expression.

Main Results:

  • BmNPV p26 transcripts and protein are detectable early in infection (3-6 hpi) and accumulate over time.
  • p26 localizes to both the cytoplasm and nucleus of infected cells.
  • RNAi-mediated knockdown of p26 significantly reduces budded virus production and downregulates late viral genes (gp64, p74).

Conclusions:

  • BmNPV p26 is an early viral gene with dual cytoplasmic and nuclear functions.
  • p26 plays a crucial role in efficient budded virus production and viral replication during the late infection stage.
Keywords:
BmNPVexpressionknockdownp26replicationsubcellular localization

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