A Simple HPLC/DAD Method Validation for the Quantification of Malondialdehyde in Rodent's Brain

Area of Science:

• Analytical Chemistry
• Neuroscience
• Biochemistry

Background:

• Malondialdehyde (MDA) is a key biomarker of lipid peroxidation and oxidative stress.
• Accurate quantification of MDA in brain tissue is crucial for understanding neurological disorders and drug effects.

Purpose of the Study:

• To develop and validate a High-Performance Liquid Chromatography with Diode Array Detection (HPLC/DAD) method for determining malondialdehyde (MDA) in rodent brains.
• To assess the method's suitability for toxicological studies involving neuroleptic drugs.

Main Methods:

• HPLC/DAD analysis using a Supelcosil LC-18 column with an acetonitrile and phosphate buffer mobile phase.
• Isocratic elution, 100 μL injection volume, 1.5 min analysis time, 1 mL/min flow rate, and detection at 532 nm.
• Validation parameters including linearity, precision, accuracy, LLOQ, stability, and robustness were evaluated.

Main Results:

• The method demonstrated good linearity (0.2-20 μg/g), acceptable precision (RSD ≤15%), and accuracy (±15%).
• Stability and robustness were confirmed, meeting validation criteria.
• The method was successfully applied to analyze MDA levels in rats treated with haloperidol and metformin.

Conclusions:

• A robust and validated HPLC/DAD method for MDA quantification in rat brains was established.
• This method is suitable for toxicological research, particularly in evaluating drug-induced oxidative stress in the brain.