Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

688
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
688
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

244
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
244
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

423
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
423

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Semisynthesis, characterisation, and antibacterial evaluation of a novel lecanoric acid-derived amide library.

Beilstein journal of organic chemistry·2026
Same author

Dual-Mode Native Mass Spectrometry Screening Identifies Ginsenoside Ligands of 6-Hydroxymethyl-7,8-Dihydropterin Pyrophosphokinase (HPPK).

Molecules (Basel, Switzerland)·2026
Same author

Targeted delivery of the BCG vaccine to dendritic cells improves protective efficacy against Mycobacterium tuberculosis.

Vaccine·2026
Same author

Robust Bioconjugated Antigens Induce Immune Responses Preventing Malaria Infection and its Transmission.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Advancing Collision-Induced Affinity Selection Mass Spectrometry for Quantitative Ligand Analysis in Complex Mixtures.

Analytical chemistry·2026
Same author

ClpC1-targeting peptide natural products differentially dysregulate the proteome of Mycobacterium tuberculosis.

Nature communications·2026

Related Experiment Video

Updated: Oct 22, 2025

Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
06:32

Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice

Published on: September 19, 2016

10.8K

Particulate Mycobacterial Vaccines Induce Protective Immunity against Tuberculosis in Mice.

Shuxiong Chen1, Diana H Quan2, Xiaonan T Wang2

  • 1Centre for Cell Factories and Biopolymers, Griffith Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia.

Nanomaterials (Basel, Switzerland)
|August 27, 2021
PubMed
Summary

New particulate vaccines using mycobacterial antigen fusions H4 and H28 show promise for tuberculosis (TB) prevention. Polyester nanoparticle-H4 demonstrated significant protection in mice, comparable to the BCG vaccine.

Keywords:
antigen nanoparticlesbioengineeringparticulate vaccinespolyester nanoparticleprotective immunityself-assemblytuberculosis

More Related Videos

Author Spotlight: Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis
06:57

Author Spotlight: Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis

Published on: May 12, 2023

2.6K
Intradermal Inoculation of Mycobacterium avium in the Mouse Ear
09:20

Intradermal Inoculation of Mycobacterium avium in the Mouse Ear

Published on: July 3, 2025

730

Related Experiment Videos

Last Updated: Oct 22, 2025

Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
06:32

Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice

Published on: September 19, 2016

10.8K
Author Spotlight: Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis
06:57

Author Spotlight: Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis

Published on: May 12, 2023

2.6K
Intradermal Inoculation of Mycobacterium avium in the Mouse Ear
09:20

Intradermal Inoculation of Mycobacterium avium in the Mouse Ear

Published on: July 3, 2025

730

Area of Science:

  • Vaccinology
  • Immunology
  • Microbiology

Background:

  • Current tuberculosis (TB) vaccines offer inconsistent protection, necessitating novel vaccine strategies.
  • Mycobacterial antigen fusions H4 and H28 are immunogenic but require effective delivery systems for enhanced efficacy.
  • Particulate delivery systems can augment the immunogenicity of soluble protein antigens.

Purpose of the Study:

  • To evaluate the immunogenicity and protective efficacy of particulate mycobacterial antigen fusions (H4 and H28) as potential TB vaccines.
  • To assess the performance of polyester nanoparticle-based vaccines compared to existing TB vaccines.

Main Methods:

  • Engineered endotoxin-free *Escherichia coli* to produce high yields of particulate vaccines (polyester nanoparticle-H4, polyester nanoparticle-H28).
  • Purification of vaccine nanoparticles and assessment of antigen-specific T cell responses in immunized mice.
  • Challenge of vaccinated mice with aerosolized virulent *Mycobacterium tuberculosis* to determine protective immunity.

Main Results:

  • Particulate vaccines induced long-lasting antigen-specific T cell responses.
  • Significant reduction (up to 0.7-log10) in *M. tuberculosis* colony-forming units (CFU) in the lungs of mice vaccinated with particulate vaccines.
  • Polyester nanoparticle-H4 provided protective immunity comparable to the *M. bovis* bacillus Calmette-Guérin (BCG) vaccine.

Conclusions:

  • Particulate formulation of mycobacterial antigen fusions H4 and H28 effectively induces protective immunity against TB in a mouse model.
  • The polyester nanoparticle-H4 vaccine is a safe, cost-effective, and promising subunit vaccine candidate for TB.
  • This approach offers a viable strategy for developing improved TB vaccines.