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Effect of various sodium taurocholate preparations on the recovery of Clostridium difficile spores
S Kamiya1, K Yamakawa, H Ogura
1Department of Bacteriology, School of Medicine, Kanazawa University, Ishikawa.
Abstract:
The effect of four sodium taurocholate preparations, which are easily available in Japan, on recovery of Clostridium difficile spores was examined. All preparations, except for one, enabled the recovery of nearly all spores counted microscopically. Moreover, by using 69 toxigenic and 34 nontoxigenic C. difficile strains, the relationship between the recovery of spores in the medium with sodium taurocholate and toxigenicity of C. difficile was analyzed. It was noted that the number of strains with recovery rate of more than 70% was greater in toxigenic strains than in nontoxigenic strains, suggesting a more abundant recovery of toxigenic C. difficile strains in the presence of sodium taurocholate.
Insights
Sodium taurocholate preparations effectively recover Clostridium difficile spores. Toxigenic strains showed higher recovery rates, suggesting its utility in studying these specific C. difficile types.
Area of Science:
- Microbiology
- Gastroenterology
Background:
- Clostridium difficile is a significant cause of infectious diarrhea.
- Accurate recovery of Clostridium difficile spores is crucial for diagnostic and research purposes.
Purpose of the Study:
- To evaluate the efficacy of readily available sodium taurocholate preparations in recovering Clostridium difficile spores.
- To investigate the correlation between sodium taurocholate-mediated spore recovery and the toxigenicity of Clostridium difficile strains.
Main Methods:
- Four different sodium taurocholate preparations were tested for their ability to recover Clostridium difficile spores.
- Microscopic spore counts were compared with recovery rates.
- 69 toxigenic and 34 nontoxigenic C. difficile strains were analyzed for recovery rates in the presence of sodium taurocholate.
Main Results:
- Three of the four sodium taurocholate preparations demonstrated high recovery rates for Clostridium difficile spores, nearing 100% of microscopically counted spores.
- A higher proportion of toxigenic Clostridium difficile strains exhibited a recovery rate exceeding 70% compared to nontoxigenic strains.
Conclusions:
- Sodium taurocholate preparations are effective for the recovery of Clostridium difficile spores.
- The enhanced recovery of toxigenic strains suggests a potential role for sodium taurocholate in selectively enriching these clinically relevant C. difficile types.