Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chondroitin sulfate-depolymerizing activity in Streptococcus intermedius and other streptococci.

E Osano1, E Hibi, M Ozeki

  • 1Department of Microbiology, School of Dentistry, Aichi-Gakuin University.

Microbiology and Immunology
|January 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of risk factors for SARS-CoV-2 infection among healthcare workers during Omicron and Delta dominance periods in Japan.

The Journal of hospital infection·2023
Same author

Germline IKAROS mutation associated with primary immunodeficiency that progressed to T-cell acute lymphoblastic leukemia.

Leukemia·2017
Same author

ZFP521 contributes to pre-B-cell lymphomagenesis through modulation of the pre-B-cell receptor signaling pathway.

Oncogene·2015
Same author

Skewed X chromosome inactivation in fraternal female twins results in moderately severe and mild haemophilia B.

Haemophilia : the official journal of the World Federation of Hemophilia·2008
Same author

A nosocomial parvovirus B19 infection-induced transient aplastic crisis in a patient with chronic renal failure.

Clinical nephrology·2006
Same author

Phagocytosis of titanium particles and necrosis in TNF-alpha-resistant mouse sarcoma L929 cells.

Toxicology in vitro : an international journal published in association with BIBRA·2003

Streptococcus intermedius strains, except one, showed chondroitin sulfate C-depolymerizing activity. This enzyme activity was only slightly detectable in these oral bacteria, unlike the strong activity seen in the control strain.

Area of Science:

  • Microbiology
  • Enzymology
  • Oral Health

Background:

  • Streptococcus intermedius is a common bacterium found in the human oral cavity.
  • Chondroitin sulfate C is a major glycosaminoglycan in cartilage and other connective tissues.
  • Enzymes capable of degrading chondroitin sulfate may play roles in bacterial pathogenicity and tissue remodeling.

Purpose of the Study:

  • To investigate the chondroitin sulfate C-depolymerizing activity of Streptococcus intermedius strains.
  • To compare the activity of oral isolates with the type strain of S. intermedius.
  • To assess the potential implications of this activity for bacterial classification and pathogenicity.

Main Methods:

  • A modified screening plate method based on Smith and Willett was used.

Related Experiment Videos

  • The study tested the type strain (ATCC 27335) and 18 human oral isolates of S. intermedius.
  • Propionibacterium acnes ATCC 11828 served as a positive control.

    • Main Results:

    • Most S. intermedius strains (except ATCC 31412) exhibited chondroitin sulfate C-depolymerizing activity.
    • The detected activity in S. intermedius was only slightly detectable.
    • Propionibacterium acnes showed strong chondroitin sulfate C-depolymerizing activity.

    Conclusions:

    • Streptococcus intermedius possesses a low level of chondroitin sulfate C-depolymerizing activity.
    • This enzymatic activity may be relevant for understanding the ecological role and pathogenic potential of S. intermedius.
    • Further research is warranted to elucidate the specific enzymes involved and their functional significance.