Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding the Impact of Hypoxia on Pulmonary Artery Endothelial Cells in Chronic Thromboembolic Pulmonary Hypertension Patients.

International journal of molecular sciences·2026
Same author

Physiomimetic culture bias durotaxis toward soft environments.

bioRxiv : the preprint server for biology·2026
Same author

Sarcopenia and postoperative outcomes in patients with peritoneal carcinomatosis undergoing cytoreductive surgery and hyperthermic intraperitoneal chemotherapy.

Abdominal radiology (New York)·2026
Same author

Development and optimization of an easy to interpret loop-mediated isothermal amplification (LAMP) assay for the identification of bacterial pathogens causing childhood pneumonia.

Frontiers in microbiology·2026
Same author

Advances in Binders for Construction Materials.

Materials (Basel, Switzerland)·2026
Same author

Precise dynamic control of tissue oxygenation during brain slice electrophysiology.

American journal of physiology. Cell physiology·2025

Related Experiment Video

Updated: Oct 22, 2025

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies
08:10

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

7.0K

Image-Based Method to Quantify Decellularization of Tissue Sections.

Maria Narciso1,2, Jorge Otero1,2,3, Daniel Navajas1,2,3

  • 1Unitat de Biofísica i Bioenginyeria, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, 08036 Barcelona, Spain.

International Journal of Molecular Sciences
|August 27, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a novel image processing algorithm for quantifying DNA in tissues, offering a faster, non-destructive alternative to traditional methods for assessing tissue decellularization.

Keywords:
decellularizationfluorescence imageimage processingmicroscopic imagesegmentation

More Related Videos

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
09:31

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Published on: April 8, 2015

11.8K
Decellularization of the Murine Cardiopulmonary Complex
08:34

Decellularization of the Murine Cardiopulmonary Complex

Published on: May 30, 2021

3.1K

Related Experiment Videos

Last Updated: Oct 22, 2025

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies
08:10

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants as 3D-like Platforms for In Vitro Studies

Published on: March 21, 2019

7.0K
Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
09:31

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

Published on: April 8, 2015

11.8K
Decellularization of the Murine Cardiopulmonary Complex
08:34

Decellularization of the Murine Cardiopulmonary Complex

Published on: May 30, 2021

3.1K

Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Molecular Biology

Background:

  • Traditional DNA quantification for tissue decellularization is destructive and unsuitable for scarce samples.
  • Existing methods require tissue digestion, limiting their application and efficiency.

Purpose of the Study:

  • To develop a faster, simpler, and more comprehensive image-based method for quantifying DNA in native and decellularized tissues.
  • To provide a non-destructive alternative to current DNA quantification techniques.

Main Methods:

  • An image processing algorithm utilizing local entropy for mask creation on phase contrast images.
  • Application of the mask to UV-labeled nuclei images to measure fluorescence intensity as a DNA content proxy.
  • Validation across lung and heart tissues, comparing results with standard DNA quantification.

Main Results:

  • The image processing algorithm accurately quantifies DNA content in (de)cellularized tissues.
  • Results correlate well with conventional DNA quantification methods.
  • The method successfully tracks DNA reduction over time during decellularization and allows for further protein analysis.

Conclusions:

  • The developed algorithm offers a robust, non-destructive, and efficient method for assessing tissue decellularization.
  • This image-based approach enhances quantitative analysis of tissue samples, especially when tissue is limited.
  • The technique facilitates comprehensive tissue characterization beyond DNA content, including protein analysis.