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Expression Profile of Genes Encoding Proteins Involved in Regulation of Vasculature Development and Heart Muscle
Mariusz J Nawrocki1, Karol Jopek2, Maciej Zdun3
1Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland.
Insights
Understanding cardiac regeneration is crucial for treating heart failure after acute coronary syndromes (ACS). This study reveals differences in gene expression between heart compartments, highlighting pathways involved in new blood vessel formation.
Area of Science:
- Cardiovascular Biology
- Molecular Cardiology
- Regenerative Medicine
Background:
- Acute coronary syndromes (ACS) often lead to heart failure with reduced ejection fraction.
- Current treatments cannot replace infarcted or dysfunctional heart muscle.
- Cardiac regeneration mechanisms, particularly new blood vessel development, are vital for recovery.
Purpose of the Study:
- To investigate gene expression patterns in cardiac cells to understand regeneration mechanisms.
- To compare gene expression between the right atrial appendage (RAA) and right atrium (RA).
- To identify genes and pathways involved in angiogenesis and cardiac repair.
Main Methods:
- Utilized microarray expression assays and RT-qPCR for gene expression analysis.
- Isolated and cultured primary cells from RAA and RA.
- Employed DAVID software for gene ontology analysis.
Main Results:
- Identified significant gene expression profiles related to angiogenesis and vasculature development.
- Found distinct differences in key transcript expression between RAA and RA cell sources.
- Observed increased expression of SFRP2, PRRX1, CCL2, IL6, and ROBO1 with extended culture.
- Noted significantly higher lysyl oxidase (LOX) gene expression in RAA cells post-cultivation.
Conclusions:
- Transcriptomic analysis reveals compartment-specific gene expression in the heart.
- Identified key genes and pathways potentially regulating cardiac regeneration and vascularization.
- Findings provide insights into cellular mechanisms underlying heart muscle repair and neovascularization.
Abstract:
Despite significant advances in treatment of acute coronary syndromes (ACS) many subjects still develop heart failure due to significantly reduced ejection fraction. Currently, there are no commonly available treatment strategies that replace the infarcted/dysfunctional myocardium. Therefore, understanding the mechanisms that control the regeneration of the heart muscle is important. The development of new coronary vessels plays a pivotal role in cardiac regeneration. Employing microarray expression assays and RT-qPCR validation expression pattern of genes in long-term primary cultured cells isolated form the right atrial appendage (RAA) and right atrium (RA) was evaluated. After using DAVID software, it indicated the analysis expression profiles of genes involved in ontological groups such as: "angiogenesis", "blood vessel morphogenesis", "circulatory system development", "regulation of vasculature development", and "vasculature development" associated with the process of creation new blood vessels. The performed transcriptomic comparative analysis between two different compartments of the heart muscle allowed us to indicate the presence of differences in the expression of key transcripts depending on the cell source. Increases in culture intervals significantly increased expression of SFRP2, PRRX1 genes and some other genes involved in inflammatory process, such as: CCL2, IL6, and ROBO1. Moreover, the right atrial appendage gene encoding lysyl oxidase (LOX) showed much higher expression compared to the pre-cultivation state.
