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Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
Published on: August 20, 2017
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Derivation of Mouse Parthenogenetic Advanced Stem Cells
Mengyi Wei1,2, Jindun Zhang1,2, Jia Liu1,2
1State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot 010020, China.
International Journal of Molecular Sciences
|August 27, 2021
Summary
Maternal genes support parthenogenetic epiblast-like stem cells (paAFSCs). These cells can be converted to naive parthenogenetic advanced stem cells (paASCs) with enhanced developmental potential, offering new insights into pluripotency and reproduction.
Area of Science:
- Stem cell biology
- Developmental biology
- Reproductive biology
Background:
- Parthenogenetic embryos are crucial for studying imprinting genes and reproductive issues.
- Epiblast stem cells (EpiSCs) are derived from blastocysts and express key pluripotency markers.
- Naive pluripotent embryonic stem cells (ESCs) are distinct from primed EpiSCs.
Purpose of the Study:
- To establish and characterize parthenogenetic epiblast-like stem cells (paAFSCs).
- To convert paAFSCs into parthenogenetic advanced stem cells (paASCs) with naive pluripotency.
- To evaluate the developmental potential of paASCs.
Main Methods:
- Culturing parthenogenetic diploid blastocysts in a defined medium with activin A and bFGF to derive paAFSCs.
- Converting paAFSCs to paASCs using BMP4, CHIR99021, and LIF.
- Assessing pluripotency markers, morphology, and in vivo developmental potential (chimerism).
Main Results:
- Established paAFSCs expressing pluripotent and germ-layer markers, similar to EpiSCs.
- Successfully converted paAFSCs to paASCs, which resemble ESCs morphologically.
- paASCs demonstrated superior in vivo developmental potential compared to paAFSCs, forming chimeras.
Conclusions:
- Maternal imprinting genes can support the derivation of parthenogenetic EpiSCs.
- Primed paAFSCs can be reprogrammed into naive paASCs.
- This study provides a novel model for studying pluripotency and early development using parthenogenetic cells.
