Long Non-Coding RNA OIP5-AS1 Inhibits the Proliferation and Migration of Esophageal Squamous Carcinoma Cells by
Qihang Yan1, Li Liu2, Han Yang2
1Department of Thoracic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310000, Zhejiang, PR China.
Abstract:
Esophageal cancer is one of the most common human malignancies and ranks sixth for global mortality; the major histological type is esophageal squamous cell carcinoma (ESCC). Here we assessed the effect of long non-coding (lnc) RNA OIP5-AS1 on the miR-30a-5p/Forkhead box protein D1 (FOXD1) axis in ESCC and investigated the underlying mechanism involving the ERK1/2 signaling pathway. lnc RNA OIP5-AS1 was highly expressed in human ESCC tissues and cells, targeted miR-30a-5p, and inhibited miR-30a-5p expression. Additionally, in human ESCC tissues, miR-30a-5p was poorly expressed, whereas FOXD1 mRNA and protein were highly expressed, with a negative correlation between miR-30a-5p and FOXD1 expression. miR-30a-5p targeted and inhibited FOXD1 expression. FOXD1 promoted the proliferation and invasion of ESCC and was related to the ERK1/2 signaling pathway; ERK1/2 inhibitors (LY-3214996) reversed the biological function of FOXD1. miR-30a-5p combined with FOXD1 regulated ERK1/2 expression and inhibited tumor growth in vivo. In this study, micro- and nano-particles were used as carriers to construct Nanocapsules carrying miR-30a-5p mimics and miR-30a-5p inhibitor through self-assembly method, so as to realize an efficient Nanocapsules delivery system of miR-30a-5p to esophageal cancer cells. It provides insights into targeted drug therapy and the development of micro- and nano-particles carriers.
Insights
Long non-coding RNA OIP5-AS1 promotes esophageal squamous cell carcinoma (ESCC) by inhibiting miR-30a-5p, which targets FOXD1. This axis influences cell proliferation, invasion, and the ERK1/2 pathway, offering potential therapeutic targets.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Esophageal squamous cell carcinoma (ESCC) is a leading cause of cancer mortality globally.
- Understanding the molecular mechanisms driving ESCC progression is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the role of long non-coding RNA OIP5-AS1 in ESCC.
- To elucidate the regulatory axis involving OIP5-AS1, miR-30a-5p, and Forkhead box protein D1 (FOXD1) in ESCC.
- To explore the involvement of the ERK1/2 signaling pathway in this axis and its therapeutic implications.
Main Methods:
- Analysis of OIP5-AS1 and miR-30a-5p expression in ESCC tissues and cells.
- Investigating the targeting relationship between OIP5-AS1 and miR-30a-5p, and miR-30a-5p and FOXD1.
- Assessing the effect of FOXD1 on ESCC cell proliferation and invasion.
- Examining the role of the ERK1/2 signaling pathway and its inhibition.
- Utilizing micro- and nano-particles for targeted delivery of miR-30a-5p mimics and inhibitors.
Main Results:
- OIP5-AS1 was highly expressed in ESCC, inhibiting miR-30a-5p.
- miR-30a-5p was poorly expressed in ESCC and targeted FOXD1, suppressing its expression.
- FOXD1 promoted ESCC proliferation and invasion, and its activity was linked to the ERK1/2 pathway.
- miR-30a-5p and FOXD1 modulated ERK1/2 signaling and inhibited tumor growth in vivo.
- An efficient nanocapsule delivery system for miR-30a-5p was developed.
Conclusions:
- The OIP5-AS1/miR-30a-5p/FOXD1 axis plays a significant role in ESCC progression.
- Targeting this axis, particularly through miR-30a-5p, holds therapeutic potential for ESCC.
- Nanoparticle-based drug delivery systems offer a promising strategy for ESCC treatment.
Related Concept Videos
01:22MicroRNAs
02:15Experimental RNAi
02:30siRNA - Small Interfering RNAs
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
02:39lncRNA - Long Non-coding RNAs
01:23RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
02:57piRNA - Piwi-interacting RNAs