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Updated: Oct 22, 2025

Polymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine?
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Autophagy Modulation and Synergistic Therapy to Combat Multidrug Resistance Breast Cancer Using Hybrid Cell Membrane

Zhi-Qiang Zhao1, Wei Song2, Xue-Qin Yan3

  • 1The Affiliated Huai'an Hospital of Xuzhou Medical University and The Second People's Hospital of Huai'an, Huai'an, 223002, P. R. China.

Journal of Biomedical Nanotechnology
|August 27, 2021
PubMed

Insights

This study introduces a novel co-delivery system (MC/D-siR) that combines anti-autophagy siRNA and doxorubicin to overcome multidrug resistance (MDR) in breast cancer. The system effectively reverses MDR by regulating autophagy and enhancing chemotherapy, improving treatment outcomes.

Area of Science:

  • Biomedical Engineering
  • Cancer Research
  • Drug Delivery Systems

Background:

  • Multidrug resistance (MDR) significantly limits chemotherapy efficacy in cancer treatment.
  • Autophagy plays a critical role in cancer progression and the development of MDR.

Purpose of the Study:

  • To develop and evaluate a sequential co-delivery system (MC/D-siR) for anti-autophagy siRNA and doxorubicin (Dox).
  • To combat multidrug-resistant (MDR) breast cancer by targeting autophagy and enhancing chemotherapy.

Main Methods:

  • Sequential construction of a co-delivery system (MC/D-siR) for siRNA and doxorubicin.
  • Transfection of MC/D-siR into Dox-resistant MCF-7 cells (MCF-7/Adr).
  • Assessment of autophagy downregulation, ATP levels, drug retention, and anticancer efficacy.

Main Results:

  • MC/D-siR effectively delivered siRNA, significantly downregulating intracellular autophagy in MCF-7/Adr cells.
  • The system reversed MDR by cutting off ATP supply and increasing doxorubicin retention.
  • MC/D-siR demonstrated superior anticancer benefits compared to single-drug platforms.

Conclusions:

  • The MC/D-siR system offers a promising strategy for MDR breast cancer treatment.
  • Combining autophagy regulation with chemotherapy via co-delivery enhances therapeutic outcomes.

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