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Analysis of Cell Cycle Position in Mammalian Cells
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Bombyx mori cell division cycle protein 37 promotes the proliferation of BmNPV

Qian Zhang1, Yun-Fei Wu1, Peng Chen1

  • 1State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China; Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing 400715, China.

Pesticide Biochemistry and Physiology
|August 27, 2021
PubMed

Insights

Cell division cycle protein 37 (Cdc37) in silkworm cells promotes Bombyx mori nucleopolyhedrovirus (BmNPV) reproduction. This molecular chaperone interacts with BmHsp90, enhancing viral proliferation.

Area of Science:

  • Molecular biology
  • Virology
  • Insect science

Background:

  • Cell division cycle protein 37 (Cdc37) is a crucial molecular chaperone involved in various cellular processes and pathogen interactions.
  • The specific role of Cdc37 in silkworm cells during Bombyx mori nucleopolyhedrovirus (BmNPV) infection remained uncharacterized.

Purpose of the Study:

  • To investigate the function of Cdc37 in silkworm cells following BmNPV infection.
  • To elucidate the interaction between BmCdc37 and BmHsp90 in the context of viral proliferation.

Main Methods:

  • Cloning and identification of the BmCdc37 gene from Bombyx mori.
  • Analysis of BmCdc37 gene expression levels post-BmNPV infection.
  • Immunofluorescence, bimolecular fluorescence complementation (BiFC), and co-immunoprecipitation (Co-IP) assays to study protein interactions.
  • Assessment of the impact of BmCdc37 and BmHsp90 on BmNPV reproduction.

Main Results:

  • The BmCdc37 gene was cloned and found to be highly conserved.
  • BmCdc37 gene expression was upregulated after BmNPV infection, mirroring BmHsp90 expression patterns.
  • BmCdc37 was confirmed to interact with BmHsp90 in silkworm cells.
  • Both BmCdc37 and BmHsp90 were found to promote BmNPV reproduction, with co-expression yielding enhanced viral proliferation.

Conclusions:

  • BmCdc37 plays a significant role in facilitating BmNPV proliferation in silkworm cells.
  • The interaction between BmCdc37 and BmHsp90 is critical for enhancing viral reproduction.
  • Understanding this interaction provides insights into viral pathogenesis and potential control strategies.
Keywords:
BmCdc37BmHsp90BmNPVBombyx mori

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