Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Crystallography02:18

X-ray Crystallography

24.6K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
24.6K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

4.2K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A user-friendly goniometer-compatible fixed-target platform for macromolecular crystallography at synchrotrons.

Journal of applied crystallography·2026
Same author

Erratum: A simple goniometer-compatible flow cell for serial synchrotron X-ray crystallography. Corrigendum.

Journal of applied crystallography·2025
Same author

Structural and biochemical basis for activity of Aspergillus nidulans α-1,3-glucanases from glycoside hydrolase family 71.

Communications biology·2025
Same author

Preface to special topic: Celebrating the work and achievements of Keith Moffat.

Structural dynamics (Melville, N.Y.)·2025
Same author

Structural basis for the prolonged photocycle of sensory rhodopsin II revealed by serial synchrotron crystallography.

Nature communications·2025
Same author

Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate.

Nature communications·2024

Related Experiment Video

Updated: Oct 22, 2025

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
07:55

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

13.5K

Advances and challenges in time-resolved macromolecular crystallography.

Gisela Brändén1, Richard Neutze2

  • 1Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.

Science (New York, N.Y.)
|August 27, 2021
PubMed
Summary

Time-resolved crystallography reveals dynamic protein structural changes. Advanced serial crystallography techniques capture ultrafast and slower conformational shifts in biological macromolecules, offering detailed chemical insights.

More Related Videos

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

14.9K
Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

9.2K

Related Experiment Videos

Last Updated: Oct 22, 2025

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
07:55

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

13.5K
Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
11:48

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

14.9K
Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
07:26

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers

Published on: February 28, 2019

9.2K

Area of Science:

  • Structural biology
  • Biochemistry
  • X-ray crystallography

Background:

  • Biological macromolecule conformational changes drive cellular reactions.
  • Time-resolved crystallography provides detailed insights into protein structural dynamics.
  • Serial crystallography at X-ray free-electron lasers (XFELs) is a key technique.

Purpose of the Study:

  • To review recent advancements in time-resolved crystallography for studying macromolecular conformational changes.
  • To highlight the application of these techniques at synchrotron sources.
  • To showcase the visualization of both ultrafast and slower biologically relevant structural dynamics.

Main Methods:

  • Utilizing serial crystallography approaches at XFELs and synchrotrons.
  • Applying time-resolved diffraction studies to protein crystals.
  • Investigating light-driven reactions and other biological processes.

Main Results:

  • Successful visualization of ultrafast structural changes in light-driven reactions.
  • Capture of biologically significant conformational changes on slower timescales.
  • Demonstration of broad applicability to diverse macromolecules, including bacteriorhodopsin and photosystem II.

Conclusions:

  • Time-resolved crystallography, particularly serial approaches, is a powerful tool for understanding macromolecular dynamics.
  • These techniques provide unprecedented detail on the structural basis of biological function.
  • The field is expanding to new sources and a wider range of biological systems.