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Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)
Miles Armstrong1, Ingo Hein2,3
1Division of Plant Sciences, School of Life Science, University of Dundee (at the James Hutton Institute), Dundee, UK.
Abstract:
Diagnostic Resistance gene enrichment Sequencing (dRenSeq) is a method for tracking and verifying the integrity of multiple functional NLR genes in complex genetic backgrounds. Traditionally, these tasks have been addressed by laborious and time-consuming PCR-based approaches or, where cognate avirulence genes are available, effector recognition studies. Both these approaches are superseded by dRenSeq in terms of cost-effectiveness, efficiency, and accuracy.
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