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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Related Experiment Video

Updated: Oct 22, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Gene Editing in Potato Using CRISPR-Cas9 Technology.

Laura Chauvin1, François Sevestre2,3, Tjaša Lukan4

  • 1IGEPP, INRAE, Institut Agro, Univ Rennes, Ploudaniel, France.

Methods in Molecular Biology (Clifton, N.J.)
|August 27, 2021
PubMed
Summary

Genome editing using CRISPR-Cas9 enables precise gene modification in potato (Solanum tuberosum). This technology facilitates targeted gene knockout for improved crop traits in elite potato cultivars.

Keywords:
Agrobacterium tumefaciensCRISPR-Cas9Gene editingHRM analysisPlant regenerationPotatoProtoplastsSingle-guide RNA

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Area of Science:

  • Plant Science
  • Genetics
  • Biotechnology

Background:

  • Cultivated potato (Solanum tuberosum) is highly heterozygous and vegetatively propagated, making trait improvement challenging.
  • The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system offers a precise and versatile tool for genome editing in plants.

Purpose of the Study:

  • To describe strategies for designing and cloning CRISPR-Cas9 constructs for potato genome editing.
  • To optimize Agrobacterium-mediated transformation and protoplast transfection for delivering CRISPR-Cas9 components.
  • To detail methods for regenerating and characterizing edited potato plants.

Main Methods:

  • Design and cloning of single-guide RNA (sgRNA) spacer sequences into CRISPR-SpCas9 plasmids.
  • Agrobacterium-mediated stable transformation and transient transfection of potato protoplasts.
  • Optimization of DNA delivery, plant regeneration, and molecular screening using PCR-based methods like high-resolution melt (HRM) analysis.

Main Results:

  • Successful gene knockout in potato via the non-homologous end-joining (NHEJ) DNA repair pathway.
  • Established protocols for efficient delivery of CRISPR-Cas9 components into potato cells.
  • Demonstrated molecular screening methods for identifying edited potato plants.

Conclusions:

  • CRISPR-Cas9 technology provides a powerful approach for targeted genome editing in elite potato cultivars.
  • Optimized transformation and regeneration protocols enhance the efficiency of generating edited potato plants.
  • The described methods facilitate the molecular characterization of gene edits in Solanum tuberosum.