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Programmable DNA Framework Sensors for In Situ Cell-Surface pH Analysis.

Jingxin Liu1, Weiwu Li2, Rongsong Li1

  • 1College of Health Science and Environmental Engineering, Shenzhen Technology University, Shenzhen 518118, China.

Analytical Chemistry
|August 27, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed programmable DNA nanosenors for precise pH monitoring in biological settings. These tunable sensors enable real-time cell surface pH analysis, aiding disease diagnostics.

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Area of Science:

  • Biotechnology
  • Nanomedicine
  • Bioanalysis

Background:

  • Developing sensors for biological environments is crucial for bioanalysis, nanomedicine, and nanorobotics.
  • Programmable sensors with defined responsiveness are needed.

Purpose of the Study:

  • To create tunable pH sensors using DNA nanotechnology for biological applications.
  • To enable in situ cell-surface pH analysis for disease diagnostics.

Main Methods:

  • Utilized a tetrahedral DNA framework (TDF) as a structural skeleton.
  • Incorporated DNA i-motif structures as proton-recognition probes.
  • Developed a two-step anchoring strategy for cell surface attachment.

Main Results:

  • Achieved fine-tuning of sensor response midpoint and dynamic range by altering i-motif sequences.
  • Created sensors with consecutively distributed pH response windows (5.0-7.5).
  • Successfully demonstrated in situ cell-surface pH analysis using the developed nanosensors.

Conclusions:

  • The programmable i-motif-TDF nanosensors offer controllable tunability for biological pH sensing.
  • This platform is valuable for diagnosing diseases linked to extracellular pH changes.
  • The developed sensors provide a robust tool for bioanalytical and nanomedical applications.