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Updated: Oct 22, 2025
Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
Published on: September 17, 2021
Characteristics of circRNAs expression profiles in the piglets intestine induced by oxidative stress
Zhi-Xin Li1, Wei Chen1, Ming Qin1
1Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, No. 61 DaiZong Street, Tai'an City, 271018, Shandong Province, People's Republic of China.
Backgroud:
Oxidative stress (OS) can affect the expression of key genes and destroy the intestinal structure. However, it is unclear how OS regulates the expression of circular RNAs (circRNAs), microRNAs (miRNAs) and mRNAs.
Objective:
The aim of this study was to examine the expression of circRNAs, miRNAs and mRNAs exposed to OS.
Methods:
Piglets were exposed to diquat (DQ), a herbicide, and the activity of antioxidant enzymes and the morphology of the intestine were investigated. We utilized whole transcriptome sequencing to examine the global expression of circRNAs, miRNAs and mRNAs in the jejunum.
Results:
Compared to controls, 751 circRNAs, 731 miRNAs and 164 mRNAs were differentially expressed in diquat-treated piglets. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that oxidative phosphorylation, RNA degradation and ubiquitin-mediated proteolysis were closely associated with OS.
Conclusions:
Our results indicated that diquat-induced OS alters the intestinal structure, resulting in the differential expression of circRNAs, miRNAs and mRNAs in the jejunum of piglets. Meanwhile, OS weakened the enzyme antioxidant system in serum of piglets. Our results provide a foundation for further studies on the mechanisms involved in the response to OS in the jejunum.
Insights
Diquat-induced oxidative stress (OS) in piglets alters intestinal structure and gene expression, affecting circular RNAs (circRNAs), microRNAs (miRNAs), and messenger RNAs (mRNAs). This study reveals molecular changes linked to OS in the jejunum.
Area of Science:
- Molecular Biology
- Genomics
- Toxicology
Background:
- Oxidative stress (OS) impacts gene expression and intestinal integrity.
- The regulatory role of OS on circRNAs, miRNAs, and mRNAs remains largely unknown.
Purpose of the Study:
- To investigate the expression patterns of circRNAs, miRNAs, and mRNAs under OS conditions.
- To explore the molecular mechanisms underlying OS-induced changes in the jejunum.
Main Methods:
- Piglets were exposed to diquat (DQ) to induce OS.
- Whole transcriptome sequencing was employed to analyze circRNA, miRNA, and mRNA expression in the jejunum.
- Antioxidant enzyme activity and intestinal morphology were assessed.
Main Results:
- Diquat exposure led to differential expression of 751 circRNAs, 731 miRNAs, and 164 mRNAs in piglet jejunum.
- Pathway analysis linked OS to oxidative phosphorylation, RNA degradation, and ubiquitin-mediated proteolysis.
- Diquat-induced OS impaired the serum antioxidant enzyme system.
Conclusions:
- Diquat-induced OS alters jejunal structure and dysregulates circRNA, miRNA, and mRNA expression in piglets.
- These findings provide insights into the molecular response to OS in the jejunum.
- The study lays groundwork for further research into OS mechanisms.
