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Related Experiment Video

Updated: Oct 22, 2025

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy

Published on: October 6, 2023

Toward High Spatially Resolved Proteomics Using Expansion Microscopy.

Lauranne Drelich¹, Soulaimane Aboulouard¹, Julien Franck¹

¹University of Lille, Inserm, CHU Lille, U1192 - Protéomique Réponse Inflammatoire Spectrométrie de Masse - PRISM, Lille, 59000, France.

Analytical Chemistry

|August 27, 2021

Summary

This summary is machine-generated.

Expansion microscopy (EM) enables nanoscale imaging. This study optimized EM for spatial proteomics, enhancing protein identification from enlarged tissue sections using SDS homogenization.

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Area of Science:

Biotechnology
Proteomics
Microscopy

Background:

Expansion microscopy (EM) offers nanoscale resolution with conventional optical microscopes.
EM involves embedding tissues in a swellable polymer for isotropic expansion.
Current EM methods face challenges with protein loss during homogenization.

Purpose of the Study:

To develop and optimize physical tissue expansion for spatially resolved, large-scale proteomics.
To establish a novel method compatible with manual microdissection and mass spectrometry (MS)-based proteomic analysis.
To address protein loss issues in EM during homogenization.

Main Methods:

Developed a modified protocol for tissue expansion compatible with microdissection.
Investigated various homogenization agents to maximize protein identification and minimize diffusion.
Utilized sodium dodecyl sulfate (SDS) as the optimal homogenization agent.

Main Results:

Successfully enlarged tissue sections more than 3-fold.
Identified up to 655 proteins from a 1 mm expanded section (equivalent to 330 μm original size).
Achieved high protein identification with SDS homogenization, minimizing protein loss.

Conclusions:

The optimized expansion microscopy protocol enables detailed spatial proteomics.
This cost-effective method allows for proteomic analysis of specific regions of interest.
Demonstrated compatibility of EM sample preparation with subsequent proteomic studies.