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Updated: Oct 22, 2025

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
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From Yeast to Mammals, the Nonsense-Mediated mRNA Decay as a Master Regulator of Long Non-Coding RNAs Functional

Sara Andjus1, Antonin Morillon2, Maxime Wery2

  • 1ncRNA, Epigenetic and Genome Fluidity, Institut Curie, PSL University, Sorbonne Université, CNRS UMR3244, 26 Rue d'Ulm, CEDEX 05, F-75248 Paris, France.

Non-Coding RNA
|August 27, 2021
PubMed

Insights

Nonsense-Mediated mRNA Decay (NMD) surveillance extends beyond faulty mRNAs to regulate normal transcripts and long non-coding RNAs (lncRNAs). Translation and NMD control lncRNA expression, impacting cellular responses to environmental shifts.

Area of Science:

  • Molecular Biology
  • RNA Metabolism
  • Gene Regulation

Background:

  • Nonsense-Mediated mRNA Decay (NMD) is traditionally known as a translation-dependent RNA surveillance pathway.
  • NMD degrades aberrant messenger RNAs (mRNAs) harboring premature termination codons.
  • Emerging evidence reveals NMD's broader roles in regulating physiological gene expression.

Purpose of the Study:

  • To review NMD activation mechanisms in yeast and mammals.
  • To discuss the molecular basis for long non-coding RNA (lncRNA) sensitivity to NMD.
  • To explore the functional implications of NMD and translation in lncRNA metabolism.

Main Methods:

  • Literature review of NMD mechanisms.
  • Analysis of NMD regulation in yeast and mammalian systems.
  • Examination of lncRNA interactions with NMD and translation machinery.

Main Results:

  • NMD regulates not only aberrant mRNAs but also normal mRNAs and lncRNAs.
  • Specific molecular features confer NMD sensitivity to lncRNAs.
  • Functional micropeptides can be produced from NMD-regulated lncRNAs.

Conclusions:

  • Translation and NMD are key regulators of lncRNA expression.
  • This regulation is crucial for cellular adaptation to environmental changes.
  • NMD's role in RNA metabolism is more diverse than previously understood.
Keywords:
Upf1lncRNAmicropeptidenonsense-mediated mRNA decaytranslation

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