Vitamin D₃ protects turbot (Scophthalmus maximus L.) from bacterial infection

Accumulating evidence supports that vitamin D₃ (VD₃) possesses immunomodulatory properties besides its classical actions in calcium and bone homeostasis. In this study, juvenile turbots were fed with the diets containing 0 IU/kg VD₃ or the optimum dose of 400 IU/kg VD₃ for 8 weeks. To investigate the effects of VD₃ on anti-infectious immunity in fish, 10⁷ CFU Edwardsiella tarda was injected intraperitoneally to each juvenile turbot after the feeding trial. Our results showed that the mortality of infected turbots with dietary VD₃ was much lower than that in VD₃ deficient group, and the supplementation of dietary VD₃ significantly reduced the bacterial load in the spleen of infected turbots. Further analysis demonstrated that the production of reactive oxygen species (ROS) in haemocytes and lysozyme activity in serum was elevated, and the responses of T cells and B cells were modulated in VD₃-supplemented turbots. Moreover, the inflammation was significantly exacerbated in the infected turbots fed with 0 IU/kg VD₃ compared to the fish fed with 400 IU/kg VD₃. In addition, the head kidney macrophages (HKMs) in turbots were isolated and incubated with VD₃in vitro, the results showed that VD₃ significantly promoted the bactericidal activity in HKMs. In conclusion, our study has shown clear evidence that VD₃ positively regulates the innate and adaptive immunity in fish, which is beneficial to the defense in fish against pathogen infection.