Nonlinear pattern and algal dual-impact in N₂O emission with increasing trophic levels in shallow lakes

Shallow lakes are considered important contributors to emissions of nitrous oxide (N₂O), a powerful greenhouse gas, in aquatic ecosystems. There is a large degree of uncertainty regarding the relationship between N₂O emissions and the progress of lake eutrophication, and the mechanisms underlying N₂O emissions are poorly understood. Here, N₂O emission fluxes and environmental variables in different lakes along a trophic state gradient in the Yangtze River basin were studied. N₂O emission fluxes were -1.0-53.0 μg m^-2 h^-1 and 0.4-102.9 μg m^-2 h^-1 in summer and winter, respectively, indicating that there was marked variation in N₂O emissions among lakes of different trophic state. The non-linear exponential model explained differences in N₂O emission fluxes by the degree of eutrophication (p < 0.01). TN and chl-a both predicted 86% of the N₂O emission fluxes in shallow lakes. The predicted N₂O emission fluxes based on the IPCC EF_5r overestimated the observed fluxes, particularly those in hyper-eutrophic lakes. These findings demonstrated that nutrient-rich conditions and algal accumulation are key factors determining N₂O emission fluxes in shallow lakes. Furthermore, this study also revealed that temperature and algae accumulation-decomposition determine an N₂O emission flux in an intricate manner. A low temperature, i.e., winter, limits algae growth and low oxygen consumption for algae decomposition. The environment leaves a high dissolved oxygen concentration, slowing down N₂O consumption as the final step of denitrification. In summer, with the oxygen consumed by excess algal decomposition, the N₂O production is limited by the complete denitrification as well as the limited substrate supply of nitrate by nitrification in hypoxic or anoxic conditions. Such cascading events explained the higher N₂O emission fluxes from shallow lakes in winter compared with summer. This trend was amplified in hyper-eutrophic shallow lakes after algal disappearance. Collectively, algal accumulation played a dual role in stimulating and impeding N₂O emissions, especially in hyper-eutrophic lakes. This study expands our knowledge of N₂O emissions from shallow lakes in which eutrophication is underway.