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Antioxidant identification using a colorimetric sensor array based on Co-N-C nanozyme
Bin Liu1, Yuting Xue2, Zeyu Gao2
1College of Life Sciences, Capital Normal University, Beijing, 100048, China; Department of Chemistry, Capital Normal University, Beijing, 100048, China.
Colloids and Surfaces. B, Biointerfaces
|August 27, 2021
Summary
This study introduces a novel sensor array using single-atom nanozymes for colorimetric antioxidant detection. The sensor effectively identifies seven common antioxidants in various samples, offering a promising diagnostic tool.
Area of Science:
- Materials Science
- Analytical Chemistry
- Biochemistry
Background:
- Antioxidants play crucial roles in human health, and their accurate detection is vital for medical diagnosis.
- Existing methods for antioxidant detection can be complex and time-consuming.
- Development of rapid, sensitive, and selective detection platforms is essential.
Purpose of the Study:
- To develop a simple and effective colorimetric sensor array for the discrimination of multiple antioxidants.
- To utilize Co-N-C single-atom nanozymes for enhanced catalytic activity in the sensing mechanism.
- To validate the sensor's performance in complex biological matrices like serum.
Main Methods:
- A sensor array was constructed using Cobalt-Nitrogen-Carbon (Co-N-C) single-atom nanozymes.
- The sensing mechanism relies on the peroxidase-like activity of Co-N-C nanozymes catalyzing the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 at two distinct pH levels.
- Antioxidant presence was detected by monitoring differential color changes and absorbance due to varying reducing abilities towards TMB oxidation products.
- Linear Discriminant Analysis (LDA) was employed for data processing and classification of antioxidants.
Main Results:
- The Co-N-C nanozyme-based sensor array successfully identified seven different antioxidants: glutathione (GSH), ascorbic acid (AA), cysteine (Cys), tannin (TA), Catechin (C), dopamine (DA), and uric acid (UA).
- The sensor demonstrated effective performance in both buffer solutions and complex biological samples, including human serum.
- Validation studies confirmed the sensor's reliability with antioxidant mixtures, varying concentrations, and in the presence of interfering substances.
Conclusions:
- The developed sensor array offers a versatile and effective strategy for the colorimetric identification of a wide range of antioxidants.
- The use of Co-N-C single-atom nanozymes provides a robust platform for sensitive and selective antioxidant sensing.
- This technology holds significant potential for broad applications in medical diagnostics and health monitoring.
