Related Experiment Video
Updated: Aug 9, 2026

Application and Methodology of the Non-destructive 19F Time-domain NMR Technique to Measure the Content in Fluorine-containing Drug Products
Published on: August 22, 2017
A complete proton and carbon-13 NMR analysis of fluocinonide, a fluorinated corticosteroid
V J Robinson1, A D Bain, C A Rodger
1Syntex Inc., Mississauga, Ontario, Canada.
Abstract:
This paper presents a complete analysis of the proton and carbon-13 NMR spectra of 21-acetoxy-6 alpha,9-difluoro-11 beta-hydroxy-16 alpha,17-(1-methylethylidene) bis-(oxy) pregna-1,4-diene-3,20-dione, a potent anti-inflammatory fluorosteroid. The 300 MHz proton spectrum was analyzed using a combination of the two-dimensional homonuclear chemical shift correlation (COSY) technique and one-dimensional NOE difference spectra. Exact coupling constants and chemical shifts were obtained by spectral simulation and iteration. The carbon-13 spectrum was assigned from the proton spectrum via a two-dimensional heteronuclear chemical shift experiment, and long-range fluorine-proton couplings were confirmed by a fully coupled heteronuclear COSY-type experiment.
More Related Videos
Related Concept Videos
Proton (¹H) NMR: Chemical Shift
Absorption signals of all the protium nuclei in a...
Carbon-13 (¹³C) NMR: Overview
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
Spectroscopy of Carboxylic Acid Derivatives
In the...
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...

