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The 21-acetylation of corticosteroids by Clostridium sporogenes
J Winter1, C H Shackleton, G N Morris
1Department of Pathology, St. Luke's-Roosevelt Hospital Center, New York, NY 10025.
Steroids
|November 1, 1986
Abstract:
A strain of Clostridium sporogenes, an anaerobic bacterium, isolated from sewage in New York City synthesizes two constitutive enzymes with action on steroid molecules: (i) an enzyme capable of selectively acetylating the 21-hydroxyl function of certain steroids and (ii) the corresponding esterase. Under our experimental conditions the enzymes have a strict structural requirement for 3-keto-4-ene and C-20-keto or 20 alpha-hydroxyl group and convert their respective substrates to a mixture of free and acetylated products.