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Related Concept Videos

DNA Isolation01:24

DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
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Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
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Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Extraction: Advanced Methods00:56

Extraction: Advanced Methods

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Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
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DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

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Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
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Updated: Oct 22, 2025

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
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Solid-phase extraction methods for nucleic acid separation. A review.

Peipei Li1,2, Menghang Li1,3, Dongmei Yue1

  • 1Liaoning Ocean and Fisheries Science Research Institute, Liaoning Academy of Agricultural Sciences, Dalian, Liaoning, 116023, P. R. China.

Journal of Separation Science
|August 28, 2021
PubMed
Summary
This summary is machine-generated.

Solid-phase extraction offers efficient nucleic acid purification with minimal sample use and pollution risk. This review explores advanced sorbents and devices for improved nucleic acid separation processes.

Keywords:
diagnostic polymerase chain reactionmagnetic nanoparticlesmagnetic-free separationnucleic acid absorbentsolid-phase extraction

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Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
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Area of Science:

  • Biochemistry and Molecular Biology
  • Separation Science
  • Bioengineering

Background:

  • Nucleic acid separation is crucial in various fields like biopharmaceuticals and diagnostics.
  • Conventional liquid-phase extraction has limitations including higher pollution risk and sample demand.
  • Solid-phase extraction (SPE) presents an efficient alternative with distinct advantages.

Purpose of the Study:

  • To systematically review solid-phase extraction methods for nucleic acid purification.
  • To explore novel sorbents and devices for enhanced nucleic acid separation.
  • To outline research progress and future directions in nucleic acid SPE.

Main Methods:

  • Review of functional materials for nucleic acid capture.
  • Analysis of magnetic and magnetic-free solid-phase extraction techniques.
  • Summary of separation device design, optimization, and high-throughput applications.

Main Results:

  • Solid-phase extraction demonstrates efficient phase separation and low pollution risk.
  • Various functional materials and magnetic/non-magnetic methods are effective for nucleic acid capture.
  • Optimized devices and high-throughput automation enhance nucleic acid separation efficiency.

Conclusions:

  • Solid-phase extraction is a highly effective method for nucleic acid purification.
  • Continued research into novel sorbents and devices promises more flexible and efficient separation.
  • Automation and optimized designs are key for advancing high-throughput nucleic acid applications.