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Rab29 Fast Exchange Mutants: Characterization of a Challenging Rab GTPase
Rachel C Gomez1, Edmundo G Vides1, Suzanne R Pfeffer2
1Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, USA.
Abstract:
Rab29 has been implicated in multiple membrane trafficking processes with no described effectors or regulating proteins. Its fast nucleotide exchange rate and inability to bind GDI in cytosol make it a unique and poorly understood Rab. Because the conventional, "GTP-locked" Rab mutation does not have the desired effect in Rab29, we present here the use of a fluorescence-based assay to characterize novel Rab29 mutants (I64T and V156G) that display faster nucleotide exchange rates, allowing for GEF-independent Rab29 activation.
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