Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intramolecular loops control SARS-CoV-2 nucleocapsid protein self-association and nucleic acid binding dependent on phosphorylation.

bioRxiv : the preprint server for biology·2026
Same author

Evolution of a fuzzy ribonucleoprotein complex in viral assembly.

eLife·2025
Same author

Development and characterisation of a thermoreversible sol-gel for intranasal delivery of MRI and PET imaging agents.

International journal of pharmaceutics·2025
Same author

"To be honest, no one cares": an ethnographic study of postpartum perceptions and practices after gestational diabetes in Vietnam.

BMC pregnancy and childbirth·2025
Same author

Explainable artificial intelligence for predicting rare earth elements leaching from secondary resources.

Journal of hazardous materials·2025
Same author

Evolution of a fuzzy ribonucleoprotein complex in viral assembly.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Oct 22, 2025

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.2K

Quantitative proteomic analysis for evaluating affinity isolation of extracellular vesicles.

Ai Nguyen1, Tingting Wang2, Illarion V Turko2

  • 1Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA.

Journal of Proteomics
|August 28, 2021
PubMed
Summary

Researchers developed a new method using affinity peptides to isolate extracellular vesicles (EVs) from human plasma. This technique enhances the purification of EVs, crucial for understanding their role in cell communication and therapeutic applications.

Keywords:
Extracellular vesiclesMultiple reaction monitoringQconCATsTargeted proteomics

More Related Videos

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

696
Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
10:09

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

7.0K

Related Experiment Videos

Last Updated: Oct 22, 2025

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.2K
Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

696
Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
10:09

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

7.0K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Extracellular vesicles (EVs) are vital for intercellular communication and hold therapeutic potential.
  • Current challenges exist in the isolation and characterization of EVs for research and clinical applications.

Purpose of the Study:

  • To develop and evaluate a novel affinity-based isolation method for extracellular vesicles (EVs) from human plasma.
  • To utilize absolute quantification and phage display screening for selecting effective EV-binding peptides.

Main Methods:

  • Screening of a phage display peptide library against EVs to identify high-affinity ligands.
  • Employing absolute quantification with mass spectrometry for selecting optimal peptide binders.
  • Evaluating the efficiency of affinity chromatography using peptide-Sepharose for EV isolation.
  • Utilizing MRM (Multiple Reaction Monitoring) quantification for EVs and non-EVs proteins.

Main Results:

  • Successful selection of peptides with high affinity to extracellular vesicles (EVs).
  • Demonstrated the efficacy of affinity chromatography with selected peptides for EV isolation.
  • Established absolute quantification as a key tool for evaluating purification efficiency.

Conclusions:

  • Peptides selected via phage display screening are effective ligands for extracellular vesicle (EV) isolation.
  • The developed affinity-based approach offers a promising strategy for purifying EVs.
  • Absolute quantification aids in developing robust protocols for EV isolation and marker identification.