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LncRNA-MSC-AS1 inhibits the ovarian cancer progression by targeting miR-425-5p

Yinling Zhao1, Donglan Yuan2, Dandan Zhu1

  • 1Department of Obstetrics and Gynaecology, Taizhou People's Hospital, 399 Hailing South Road, Taizhou, 225300, Jiangsu, China.

Journal of Ovarian Research
|August 29, 2021
PubMed
Abstract

Insights

Long non-coding RNA MSC-AS1 inhibits ovarian cancer progression by sponging microRNA-425-5p. This interaction suppresses cell proliferation and promotes apoptosis, offering a potential therapeutic target for ovarian cancer treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Aberrant expression of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) is linked to ovarian cancer pathogenesis.
  • The specific role and regulatory mechanisms of lncRNA MSC-AS1 in ovarian cancer remain unclear.

Purpose of the Study:

  • To investigate the role of lncRNA MSC-AS1 in ovarian cancer.
  • To elucidate the regulatory mechanism of MSC-AS1 involving microRNA-425-5p in ovarian cancer.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess MSC-AS1 and miR-425-5p expression in ovarian cancer tissues and cell lines.
  • MTT, colony formation, and flow cytometry assays to evaluate MSC-AS1 function on cell proliferation, cell cycle, and apoptosis.
  • Western blot for protein expression analysis and dual-luciferase reporter assay to confirm the targeting relationship between MSC-AS1 and miR-425-5p.

Main Results:

  • MSC-AS1 was lowly expressed, while miR-425-5p was highly expressed in ovarian cancer tissues and cells.
  • Overexpression of MSC-AS1 inhibited ovarian cancer cell proliferation and induced apoptosis in SKOV3 and A2780 cells.
  • MSC-AS1 directly targeted and negatively regulated miR-425-5p; miR-425-5p upregulation partially reversed the tumor-suppressive effects of MSC-AS1.

Conclusions:

  • MSC-AS1 acts as a tumor suppressor in ovarian cancer by sponging miR-425-5p, thereby inhibiting cancer progression.
  • The MSC-AS1/miR-425-5p axis represents a potential therapeutic target for ovarian cancer treatment.
Keywords:
ApoptosisLncRNA MSC-AS1MicroRNA-425-5pOvarian cancerProliferation

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