Hydrogen sulfide detection and zebrafish imaging by a designed sensitive and selective fluorescent probe based on...

A new long-wavelength fluorescent probe 1 that could specifically identify H₂S has been successfully synthesized and applied for imaging H₂S in zebrafish. Probe 1 was readily prepared by featuring nitrobenzene as the recognition unit coupled to resorufin. The fluorescence off-on response is based on the fact that H₂S can reduce the nitro group to an amino group, followed by the 1,6-rearrangement-elimination and the release of resorufin. By evaluating the application abilities of probe 1 in vivo and vitro, it is shown that probe 1 has high sensitivity and selectivity to H₂S, low background fluorescence interference, with a low detection limit of 17.30 μM. Notably, the occurrence of the reaction can be observed by the naked eye, and the color of the solution changes from yellow to pink. More importantly, it is the first time that using paper chips as carrier to detect H₂S, which lays a foundation for the practical application of detecting H₂S. The excellent analysis and application capabilities of probe 1 make it an effective tool for further application in practice.