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Published on: September 4, 2012
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Localized and Systemic Inflammatory Mediators in a Murine Acute Mastitis Model
Miyuki Takashima1,2, Christian Lalonde1, Laura Ashley Olszanski1
1Department of Animal and Veterinary Sciences, University of Vermont, Burlington, VT, 05405, USA.
Journal of Inflammation Research
|August 30, 2021
Summary
Endotoxin challenge in the mammary gland triggers inflammation, oxidative stress, and altered metabolism, leading to milk depression. These findings identify potential targets for treating gram-negative bacteria-induced mastitis in dairy animals.
Area of Science:
- Veterinary Immunology
- Molecular Biology
- Dairy Science
Background:
- Milk depression is a significant economic issue in dairy animals, primarily driven by mastitis.
- Understanding the molecular mechanisms behind milk depression is crucial for developing effective treatments.
Purpose of the Study:
- To identify mediators of milk depression by examining local and systemic changes in gene expression and cytokine production.
- To investigate mammary gland responses to endotoxin challenge in a mouse model.
Main Methods:
- Mouse mammary glands were challenged with lipopolysaccharide (LPS) or sterile phosphate-buffered saline (PBS).
- Gene expression was analyzed using RNA sequencing and real-time PCR.
- Plasma cytokine levels were measured using a multiplex assay.
Main Results:
- LPS challenge induced pro-inflammatory responses, oxidative stress, and altered metabolic processes in the mammary gland.
- Systemic analysis revealed increased levels of 24 out of 32 cytokines, including IL-6 and TNFα.
- Local analysis showed increased levels of IL-5, IL-6, MCP-1, and TNFα in the mammary gland.
Conclusions:
- The study identified key inflammatory mediators and metabolic alterations associated with endotoxin-induced milk depression.
- These findings offer potential biomarkers and therapeutic targets for gram-negative bacteria-induced mastitis.
