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Glial Cells

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Related Experiment Video

Updated: Oct 22, 2025

Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

Published on: June 4, 2020

Astrocyte Heterogeneity in Multiple Sclerosis: Current Understanding and Technical Challenges.

Chih Hung Lo¹, Mario Skarica², Mohammad Mansoor¹

¹Department of Neurology, Yale School of Medicine, New Haven, CT, United States.

Frontiers in Cellular Neuroscience

|August 30, 2021

Summary

Single-cell technologies reveal astrocyte subpopulations in multiple sclerosis (MS) tissue. These advanced methods help understand glial phenotypes driving MS progression and disease subtypes.

Keywords:

astrocytes experimental autoimmune encephalomyelitis multiple sclerosis multiplexed imaging single nucleus sequencing

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Last Updated: Oct 22, 2025

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Published on: June 4, 2020

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Study Glial Cell Heterogeneity Influence on Axon Growth Using a New Coculture Method

Study Glial Cell Heterogeneity Influence on Axon Growth Using a New Coculture Method

Published on: September 6, 2010

Area of Science:

Neuroimmunology
Cellular Biology
Computational Biology

Background:

Multiple sclerosis (MS) involves complex immune and central nervous system interactions.
Astrocyte heterogeneity is increasingly recognized but challenging to resolve in MS.
Understanding glial cell dynamics is crucial for MS pathogenesis.

Purpose of the Study:

To review current knowledge of astrocyte subpopulations in MS.
To discuss challenges in resolving astrocyte heterogeneity using single-nucleus RNA-sequencing (snRNA-seq).
To explore multiplexed imaging for spatial context of cell populations.

Main Methods:

Review of current literature on single-cell technologies in MS research.
Discussion of single-nucleus RNA-sequencing (snRNA-seq) for astrocyte heterogeneity.
Exploration of multiplexed imaging techniques for spatial cell profiling.

Main Results:

Single-cell technologies offer unprecedented depth in characterizing immune/CNS cell assemblies in MS.
snRNA-seq presents challenges in fully resolving astrocyte heterogeneity.
Multiplexed imaging provides spatial context for cell population clusters.

Conclusions:

Single-cell and multiplexed imaging technologies are vital for advancing MS research.
These methods can elucidate glial phenotypes driving MS progression.
Future applications may clarify neuropathological differences across MS subtypes.