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Published on: August 17, 2019
Quantitative Structure-Activity Relationship Modeling of the Amplex Ultrared Assay to Predict Thyroperoxidase
Domenico Gadaleta1, Luca d'Alessandro1, Marco Marzo1
1Laboratory of Environmental Chemistry and Toxicology, Department of Environmental Health Sciences, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milan, Italy.
Abstract:
The thyroid system plays a major role in the regulation of several physiological processes. The dysregulation of the thyroid system caused by the interference of xenobiotics and contaminants may bring to pathologies like hyper- and hypothyroidism and it has been recently correlated with adverse outcomes leading to cancer, obesity, diabetes and neurodevelopmental disorders. Thyroid disruption can occur at several levels. For example, the inhibition of thyroperoxidase (TPO) enzyme, which catalyses the synthesis of thyroid hormones, may cause dysfunctions related to hypothyroidism. The inhibition of the TPO enzyme can occur as a consequence of prolonged exposure to chemical compounds, for this reason it is of utmost importance to identify alternative methods to evaluate the large amount of pollutants and other chemicals that may pose a potential hazard to the human health. In this work, quantitative structure-activity relationship (QSAR) models to predict the TPO inhibitory potential of chemicals are presented. Models are developed by means of several machine learning and data selection approaches, and are based on data obtained in vitro with the Amplex UltraRed-thyroperoxidase (AUR-TPO) assay. Balancing methods and feature selection are applied during model development. Models are rigorously evaluated through internal and external validation. Based on validation results, two models based on Balanced Random Forest (BRF) and K-Nearest Neighbours (KNN) algorithms were selected for a further validation phase, that leads predictive performance (BA = 0.76-0.78 on external data) that is comparable with the reported experimental variability of the AUR-TPO assay (BA ∼0.70). Finally, a consensus between the two models was proposed (BA = 0.82). Based on the predictive performance, these models can be considered suitable for toxicity screening of environmental chemicals.
Insights
Quantitative structure-activity relationship (QSAR) models were developed to predict the thyroid-disrupting potential of chemicals by inhibiting thyroperoxidase (TPO). These models offer reliable toxicity screening for environmental pollutants.
Area of Science:
- Environmental Toxicology
- Computational Chemistry
- Endocrinology
Background:
- Thyroid system disruption by xenobiotics is linked to various pathologies, including cancer, obesity, and neurodevelopmental disorders.
- Thyroid disruption can occur via inhibition of thyroperoxidase (TPO), an enzyme crucial for thyroid hormone synthesis.
- Identifying chemicals that inhibit TPO is vital for human health risk assessment.
Purpose of the Study:
- To develop and validate quantitative structure-activity relationship (QSAR) models for predicting the TPO inhibitory potential of chemicals.
- To establish reliable computational tools for screening environmental pollutants and chemicals for thyroid-disrupting activity.
Main Methods:
- Development of QSAR models using machine learning algorithms (Balanced Random Forest and K-Nearest Neighbours) based on *in vitro* Amplex UltraRed-thyroperoxidase (AUR-TPO) assay data.
- Application of balancing methods and feature selection during model development.
- Rigorous internal and external validation of the developed models, including a consensus model.
Main Results:
- Two QSAR models (BRF and KNN) achieved predictive performance (balanced accuracy of 0.76-0.78) comparable to experimental variability (BA ~0.70) on external data.
- A consensus model combining BRF and KNN demonstrated enhanced predictive performance (BA = 0.82).
- The validated models are suitable for the toxicity screening of environmental chemicals.
Conclusions:
- QSAR models provide a reliable and efficient approach for predicting TPO inhibitory potential.
- These computational tools can aid in the early identification of thyroid-disrupting chemicals, supporting environmental and human health protection.
- The developed models represent a significant advancement in *in silico* toxicology for endocrine disruptor assessment.
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