Related Experiment Video
Updated: Oct 22, 2025
08:23
Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
Published on: March 2, 2020
13.0K
Strategies of Detecting Bacteria Using Fluorescence-Based Dyes
Shin A Yoon1, Sun Young Park1, Yujin Cha1
1Department of Chemistry, Sookmyung Women's University, Seoul, South Korea.
Frontiers in Chemistry
|August 30, 2021
Summary
Recent advances in fluorescence-based dyes enable effective bacterial detection and imaging for theranostics. These probes offer real-time analysis and antimicrobial effects, crucial for developing new antibiotics and treating infections.
Area of Science:
- Biomedical Engineering
- Microbiology
- Analytical Chemistry
Background:
- Bacterial infection theranostics and antibiotic development require precise bacterial identification.
- Conventional detection methods have limitations, driving the need for advanced techniques.
- Organic fluorescent probes offer sensitive, real-time detection and imaging capabilities.
Purpose of the Study:
- To review recent advancements in fluorescence-based dyes for bacterial detection.
- To outline various detection strategies utilizing these probes.
- To discuss the potential of these probes in theranostics and antimicrobial agent development.
Main Methods:
- Review of literature on fluorescence-based bacterial detection strategies.
- Categorization of detection mechanisms: cell wall interactions, enzyme reactions, and peptidoglycan synthesis.
- Analysis of theranostic probes with simultaneous detection and photodynamic antimicrobial effects.
Main Results:
- Fluorescence probes enable "off-on" detection for real-time in vitro and in vivo imaging.
- Strategies include targeting bacterial components and enzymatic activity.
- Validated probes show efficacy against diverse bacteria, including antibiotic-resistant strains, in various biological models.
Conclusions:
- Fluorescence-based probes are powerful tools for bacterial detection and theranostics.
- Further research is essential for developing effective in vitro and in vivo detection methods.
- These probes hold significant promise for advancing antimicrobial agent development and infection treatment.
Related Concept Videos
01:12Immunofluorescence Microscopy
12.0K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
12.0K
03:31Labeling DNA Probes
8.6K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.6K
01:13Special Staining Techniques
696
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
696
01:28Methods of Classification and Identification
395
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
395
01:26Differential Staining Technique
1.1K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
1.1K
01:03Fixation and Sectioning
6.7K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
6.7K