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Protein Expression Protocol for the Catalytic Domain of an Adenylate Cyclase Membrane-Anchored by a Vibrio Quorum
Stephanie Beltz1, Joachim E Schultz1
1Pharmazeutisches Institut der Universität Tübingen, Pharmazeutische Biochemie, Tübingen, Germany.
Bio-Protocol
|August 30, 2021
Abstract:
The direct regulation of a mycobacterial adenylate cyclase (Rv1625c) via exchange of its membrane anchor by the quorum sensing receptor CqsS (Vibrio harveyi) has recently been reported ( Beltz et al., 2016 ). This protocol describes the expression and membrane preparation for these chimeric proteins.

