Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

KDM5 demethylases suppress R-loop-mediated 'viral mimicry' and DNA damage in breast cancer cells.

eLife·2025

Same author

Spontaneous Fusion with Transformed Mesenchymal Stromal Cells Results in Complete Heterogeneity in Prostate Cancer Cells.

Cancers·2024

Same author

A cisplatin conjugate with tumor cell specificity exhibits antitumor effects in renal cancer models.

BMC cancer·2023

Same author

The driver role of JAK-STAT signalling in cancer stemness capabilities leading to new therapeutic strategies for therapy- and castration-resistant prostate cancer.

Clinical and translational medicine·2022

Same author

Application of Pharmacokinetic/Pharmacodynamic Modeling to Bridge Mouse Antitumor Efficacy and Monkey Toxicology Data for Determining the Therapeutic Index of an Interleukin-10 Fc Fusion Protein.

Frontiers in pharmacology·2022

Same author

Target-Mediated Drug Disposition Affects the Pharmacokinetics of Interleukin-10 Fragment Crystallizable Fusion Proteins at Pharmacologically Active Doses.

Drug metabolism and disposition: the biological fate of chemicals·2022

Search research articles

Related Experiment Video

Updated: Oct 22, 2025

Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression

Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression

Published on: October 6, 2023

miRNA Characterization from the Extracellular Vesicles.

Sajni Josson^1,2, Murali Gururajan^1,1,3, Leland W K Chung¹

¹Uro-Oncology Research Program, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, USA.

|August 30, 2021

Summary

This summary is machine-generated.

Stromal fibroblasts secrete specific microRNAs (miRNAs) within extracellular vesicles that promote prostate cancer growth. These miRNAs activate pathways leading to tumor progression and metastasis, offering potential diagnostic biomarkers.

Keywords:

Biofluids Biomarkers Cancer Cancer associated stroma Exosomes Extracellular vesicles Quantitative real-time PCR miRNA

More Related Videos

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Published on: November 8, 2024

Related Experiment Videos

Last Updated: Oct 22, 2025

Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression

Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression

Published on: October 6, 2023

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

Published on: November 8, 2024

Area of Science:

Oncology
Molecular Biology
Biochemistry

Background:

Extracellular vesicles (EVs) mediate intercellular communication in cancer.
MicroRNAs (miRNAs) are key regulators of cancer progression and metastasis.
miRNAs are stable in biofluids and serve as potential disease biomarkers.

Purpose of the Study:

To identify and characterize specific miRNAs secreted by prostate cancer-associated stromal cells.
To investigate the role of these miRNAs in promoting cancer cell growth and metastasis.
To establish a method for quantifying these miRNAs in EVs.

Main Methods:

Isolation of extracellular vesicles from conditioned media of prostate cancer stromal fibroblasts.
RNA purification from the vesicular fraction.
Reverse transcription and real-time PCR quantification of specific miRNAs (miR-409-3p and miR-409-5p).

Main Results:

Identified miR-409-3p and miR-409-5p secreted by activated stromal fibroblasts.
Demonstrated uptake of these miRNAs by cancer cells.
Showed that these miRNAs induce tumor growth via epithelial to mesenchymal transition (EMT).

Conclusions:

Prostate cancer stromal cells secrete EVs containing specific miRNAs (miR-409-3p/5p) that drive cancer progression.
These miRNAs activate EMT in cancer cells, promoting tumor growth and metastasis.
The identified miRNAs are potential biomarkers for prostate cancer diagnosis and prognosis.