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Updated: Oct 22, 2025
Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms
Published on: March 1, 2022
Pathogenicity Assay of Verticillium nonalfalfae on Hop Plants
Marko Flajšman1, Sebastjan Radišek2, Branka Javornik1
1Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Abstract:
Verticillium nonalfalfae is a soil-borne plant pathogen that infects its hosts through roots. It spreads in the plant's xylem and causes wilt disease symptoms by secreting different virulence factors. Hop (Humulus lupulus) is a primary host of V. nonalfalfae, so it is used as a model plant for studying this phytopathogenic fungus. Artificial infections of hop plants and disease scoring are prerequisites for studying the pathogen's virulence/pathogenicity and its interaction with hop plants. In this protocol, we describe the root dipping inoculation method for conducting pathogenicity assay of V. nonalfalfae on hop plants.
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