Related Experiment Video
Updated: Oct 22, 2025

Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method
Published on: October 23, 2013
Xylem Sap Extraction Method from Hop Plants
Marko Flajšman1, Stanislav Mandelc1, Sebastjan Radišek2
1Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Abstract:
Verticillium wilt is one of the most important diseases on hop that significantly influence continuation of production on affected areas. It is caused by the soil borne vascular pathogen Verticillium nonalfalfae, which infects plants through the roots and then advances through the vascular (xylem) system. During infection, V. nonalfalfae secretes many different virulence factors. Xylem sap of infected plants is therefore a rich source for investigating the molecules that are involved in molecular interactions of Verticillium - hop plants. This protocol provides instructions on how to infect hop plants with V. nonalfalfae artificially and how to obtain xylem sap from hop plants.
Related Concept Videos
Xylem and Transpiration-driven Transport of Resources
Phloem and Sugar Transport
The Apoplast and Symplast
Short-distance Transport of Resources

