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Related Experiment Video

Updated: Oct 22, 2025

Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method

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Published on: October 23, 2013

Xylem Sap Extraction Method from Hop Plants.

Marko Flajšman¹, Stanislav Mandelc¹, Sebastjan Radišek²

¹Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.

|August 30, 2021

Summary

Verticillium wilt, a significant hop disease caused by Verticillium nonalfalfae, impacts crop production. This study details methods for artificial plant infection and xylem sap collection for research.

Keywords:

Extraction method Molecular interactions Vascular pathogen Verticillium nonalfalfae Xylem sap

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Area of Science:

Plant Pathology
Agricultural Science
Molecular Biology

Background:

Verticillium wilt significantly impacts hop production.
The disease is caused by the soil-borne vascular pathogen Verticillium nonalfalfae.
Infection occurs via roots, with the pathogen colonizing the xylem.

Purpose of the Study:

To provide a protocol for artificial infection of hop plants with Verticillium nonalfalfae.
To outline methods for collecting xylem sap from infected hop plants.
To facilitate research into the molecular interactions between Verticillium and hop plants.

Main Methods:

Artificial inoculation of hop plants with Verticillium nonalfalfae.
Isolation and collection of xylem sap from infected hop plants.
Detailed procedural steps for infection and sap extraction.

Main Results:

Successful artificial infection of hop plants was achieved.
A reliable method for obtaining xylem sap from infected plants was established.
The collected xylem sap serves as a source for identifying virulence factors.

Conclusions:

The protocol enables controlled study of Verticillium wilt in hops.
Xylem sap analysis can reveal key molecules in host-pathogen interactions.
This method supports research into disease resistance and management strategies.