Functional kupffer cells migrate to the liver from the intraperitoneal cavity
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Updated: Oct 22, 2025
Kupffer Cell Isolation for Nanoparticle Toxicity Testing
Published on: August 18, 2015
Wen-Ling Lin1, Mizuki Mizobuchi1, Mina Kawahigashi1
1Institute for Health Sciences, Tokushima Bunri University, 180 Nishihama-bouji, Yamashiro-cho, Tokushima, 770-8514, Japan.
Abstract:
We established a method of KC transplantation by intraperitoneal (i.p.) injection using EGFP-expressing cells (EGFP-KCs) and normal KCs. The novel method is easier and less invasive than conventional methods so that it is not only technically advantageous but also ethically preferable for experiments using animals. We demonstrated that KCs migrated to the liver following i.p. Injection. Engraftment in the liver was not observed for peritoneal macrophages (pMPs). This suggests that KCs migrate to the liver via a sorting mechanism. KC injection decreased the KC number at 24 h and then recovered the KCs at 10 days to a normal level. Additionally, recovery to the normal level by KC injection was observed in mice with KC depletion induced by GdCl3. These results suggest that a regulatory mechanism exists for controlling the number of KCs.
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