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T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

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Related Experiment Video

Updated: Oct 22, 2025

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

Published on: November 28, 2017

A T-cell-based immunogenicity protocol for evaluating human antigen-specific responses.

Cansu Cimen Bozkus¹, Ana Belen Blazquez^1,2, Tomohiro Enokida^1,3

¹Department of Hematology and Medical Oncology, Icahn School of Medicine at Mount Sinai, New York, NY 10127, USA.

|August 30, 2021

Summary

This summary is machine-generated.

This study presents a fast, sensitive method to expand antigen-specific T-cells from blood samples. This technique aids in identifying T-cell immunity and potential targets for new therapies.

Keywords:

Cell culture Cell-based Assays Flow Cytometry/Mass Cytometry High Throughput Screening Immunology

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Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

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Published on: May 17, 2019

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Understanding the T-cell repertoire's antigen specificities is crucial for assessing immune responses.
Identifying T-cell specificities can reveal targets for novel T-cell-based immunotherapies.

Purpose of the Study:

To develop a rapid, sensitive, and high-throughput protocol for expanding antigen-specific T-cells.
To enable the detection of antigen-specific effector cytokine formation.
To facilitate the study of T-cell immunity in various clinical and research settings.

Main Methods:

Expansion of antigen-specific T-cells from human peripheral blood mononuclear cells (PBMCs) in vitro.
Peptide stimulation to induce T-cell activation and expansion.
Flow cytometry to detect antigen-specific effector cytokine production.

Main Results:

A robust protocol for isolating and expanding antigen-specific T-cells was established.
The method demonstrated sensitivity and high-throughput capability.
The protocol allows for the analysis of T-cell responses to specific antigens.

Conclusions:

The described protocol offers an efficient approach to characterize the T-cell repertoire.
This method can be applied to screen for T-cell immunity and identify therapeutic targets.
The technique is adaptable for analyzing T-cell subsets from diverse human tissues.