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Updated: Oct 22, 2025

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions
Published on: January 7, 2019
Systematic study on the relationship between particulate matter and microbial counts in hospital operating rooms
Huiyi Tan1, Keng Yinn Wong2,3, Bemgba Bevan Nyakuma4
1School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia.
Abstract:
In this study, a systematic procedure for establishing the relationship between particulate matter (PM) and microbial counts in four operating rooms (ORs) was developed. The ORs are located in a private hospital on the western coast of Peninsular Malaysia. The objective of developing the systematic procedure is to ensure that the correlation between the PMs and microbial counts are valid. Each of the procedures is conducted based on the ISO, IEST, and NEBB standards. The procedures involved verifying the operating parameters are air change rate, room differential pressure, relative humidity, and air temperature. Upon verifying that the OR parameters are in the recommended operating range, the measurements of the PMs and sampling of the microbes were conducted. The TSI 9510-02 particle counter was used to measure three different sizes of PMs: PM 0.5, PM 5, and PM 10. The MAS-100ECO air sampler was used to quantify the microbial counts. The present study confirms that PM 0.5 does not have an apparent positive correlation with the microbial count. However, the evident correlation of 7% and 15% were identified for both PM 5 and PM 10, respectively. Therefore, it is suggested that frequent monitoring of both PM 5 and PM 10 should be practised in an OR before each surgical procedure. This correlation approach could provide an instantaneous estimation of the microbial counts present in the OR.
Insights
Monitoring particulate matter (PM) in operating rooms (ORs) is crucial. This study found PM 5 and PM 10 correlate with microbial counts, suggesting regular monitoring for better OR hygiene.
Area of Science:
- Environmental Science
- Microbiology
- Healthcare Engineering
Background:
- Maintaining sterile operating room (OR) environments is critical for patient safety.
- Particulate matter (PM) and microbial contamination are key factors affecting OR air quality.
- Standardized procedures are needed to validate the relationship between PM and microbial levels.
Purpose of the Study:
- To develop and validate a systematic procedure for assessing the correlation between particulate matter (PM) and microbial counts in hospital operating rooms (ORs).
- To ensure the reliability of measurements for PM and microbial contamination in ORs.
- To establish a basis for real-time estimation of microbial load in ORs.
Main Methods:
- Procedures were developed adhering to ISO, IEST, and NEBB standards.
- Key operating room parameters (air change rate, differential pressure, humidity, temperature) were verified.
- Particulate matter (PM 0.5, PM 5, PM 10) was measured using a TSI 9510-02 particle counter.
- Microbial counts were quantified using a MAS-100ECO air sampler.
Main Results:
- PM 0.5 showed no significant positive correlation with microbial counts.
- A 7% correlation was observed between PM 5 and microbial counts.
- A 15% correlation was identified between PM 10 and microbial counts.
Conclusions:
- PM 5 and PM 10 levels are correlated with microbial counts in operating rooms.
- Frequent monitoring of PM 5 and PM 10 in ORs before procedures is recommended.
- This correlation approach offers a potential method for instantaneous estimation of OR microbial load.
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