Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Pleural Well-differentiated Papillary Mesothelial Tumor Presenting with  Recurrent Pneumothorax and Pleural Effusion: A Case Report].

Zhongguo fei ai za zhi = Chinese journal of lung cancer·2026

Same author

Centering the marginalized: AI-driven strategies for advancing health equity in rare disease care.

Patterns (New York, N.Y.)·2026

Same author

Fight for the People's Health: The Application of Al Multiagent Systems in Medical Consortia.

Health care science·2026

Same author

The zinc finger gene SQSTM1/p62 is involved in the response to ammonia nitrogen stress in the razor clam (Sinonovacula constricta).

Developmental and comparative immunology·2026

Same author

Application value and clinical effect analysis of suspension technique in difficult thoracoscopic lobectomy: A retrospective study.

Current problems in surgery·2026

Same author

Clinical Application of Progressive Suspension in Thoracoscopic Right Upper Lobectomy.

ANZ journal of surgery·2026

Search research articles

Related Experiment Video

Updated: Oct 22, 2025

The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria

The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria

Published on: May 3, 2021

[Study on the temperature characteristics of fast capacitance in patch clamp experiments].

Fanyi Kong¹, Xinyu Li², Ruonan Jiao¹

¹Biomedical Optics Laboratory, School of Optoelectronic Engineering and Instrumentation Science, Dalian University of Technology, Dalian, Liaoning 116000, P.R.China.

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi = Journal of Biomedical Engineering = Shengwu Yixue Gongchengxue Zazhi

|August 30, 2021

Summary

Patch clamp electrophysiology requires accurate capacitance compensation. This study reveals how temperature affects fast capacitance, primarily by altering pipette solution resistance, crucial for precise cellular recordings.

Keywords:

fast capacitance fast capacitance compensation fast capacitance temperature characteristic patch clamp

More Related Videos

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

Published on: January 19, 2012

Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell

Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell

Published on: October 22, 2014

Related Experiment Videos

Last Updated: Oct 22, 2025

The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria

The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria

Published on: May 3, 2021

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

Published on: January 19, 2012

Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell

Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell

Published on: October 22, 2014

Area of Science:

Cellular electrophysiology
Biophysics
Membrane biophysics

Background:

Patch clamp technique is vital for cellular electrophysiology, measuring picoampere currents.
Accurate measurements necessitate compensation for pipette resistance and capacitance (slow and fast).
Temperature characteristics of capacitance require further investigation for precise electrophysiological studies.

Purpose of the Study:

To investigate the temperature-dependent characteristics of fast capacitance in patch clamp recordings.
To propose an equivalent circuit model for calculating temperature-dependent parameters.
To elucidate the mechanism by which bath solution temperature influences fast capacitance.

Main Methods:

Utilized patch clamp technique to record cellular electrophysiology.
Studied the photothermal effect on cell membranes.
Developed and applied an equivalent circuit model to analyze temperature-dependent capacitance and resistance.

Main Results:

Observed that the time constant of fast capacitance discharge changes with increasing bath solution temperature.
Quantified fast capacitance increase at 0.04 pF/℃ and a decrease in pipette resistance with rising temperature.
Demonstrated that elevated bath solution temperatures primarily affect fast capacitance kinetics by altering the inner solution resistance of the glass pipette.

Conclusions:

Temperature significantly influences fast capacitance in patch clamp recordings.
The primary driver of temperature-dependent fast capacitance is the change in pipette solution resistance.
Findings provide essential reference for precise temperature characteristic studies in cellular electrophysiology using patch clamp.