Warfarin Induced Quenching of the Carbon Quantum Dots: Mechanism Study and Warfarin Sensor Construction

The mechanism of the fluorescence quenching of the CQDs by warfarin was determined and based on this study a simple, low cost and highly sensitive nanosensor was developed for determination of Warfarin in plasma samples. The carbon quantum dots with 3.5 µs lifetime (halflife of 2.4 µs) were synthesized by hydrothermal method and characterized. The fluorescence rate constant of 4.5 × 10⁴ s^-1 and quenching rate constant of 6.18 × 10⁴ s^-1 (from 10 μM warfarin that result in 17% lifetime reduction) was calculated. High quenching efficiency results in 21.63 L mmol^-1 Stern-Volmer constant and the study of pH and temperature also confirm the dynamic quenching mechanism. The second order rate constant of 6.18 × 10⁴ L mmol^-1 s^-1 was obtained for collisions between CQDs and warfarin. Based on this mechanism, a simple, low cost and very sensitive warfarin nanosensor was developed with calibration sensitivity of 21.63 L mmol^-1, working range of 0.10 - 12.00 μM and detection limit of 0.01 μM.