Cancer associated mutations in Sec61γ alter the permeability of the ER translocase
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View abstract on PubMed
Summary
This summary is machine-generated.Mutations in the Sec61 complex
Area Of Science
- Molecular Biology
- Cell Biology
- Biochemistry
Background
- The Sec61 complex facilitates protein translocation across the ER membrane.
- It also maintains the ER permeability barrier, crucial for cellular function.
- Disruptions in Sec61 integrity are linked to disease progression.
Purpose Of The Study
- To investigate the functional impact of Sec61γ/Sss1p mutations found in cancer.
- To determine if these mutations affect the gating of the ER translocon.
Main Methods
- Analysis of cancer genome database for Sec61γ/Sss1p mutations.
- Functional assessment of identified mutations on translocon gating dynamics.
Main Results
- Six mutations in highly conserved amino acids of Sec61γ/Sss1p were identified.
- Five of these mutations were found to significantly affect ER translocon gating.
- The degree of gating alteration varied among the identified mutations.
Conclusions
- Mutations in Sec61γ occurring in malignant cells alter translocon gating dynamics.
- The ER translocon represents a potential therapeutic target for cancer co-therapy.
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