Related Experiment Video
Updated: Jul 4, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Alkaline pretreatment and response surface methodology based recombinant enzymatic saccharification and fermentation
Kaustubh Chandrakant Khaire1, Vijayanand Suryakant Moholkar2, Arun Goyal3
1School of Energy Science and Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
Abstract:
In present study, the water-soluble extractives removal prior to alkali pretreatment of sugarcane tops (SCT) was carried out. The solid alkali pretreated SCT (apSCT) recovered on Field-emission scanning electron microscopy (FE-SEM) analysis showed exposure of cellulosic fibres as compared with raw SCT. The analyses of apSCT by Fourier Transform Infrared (FT-IR) Spectroscopy, X-ray diffraction (XRD) and High performance liquid chromatography (HPLC) analysis also confirmed the enhanced cellulose content in apSCT. Optimum conditions for response surface methodology based saccharification of apSCT at 40 °C, 150 rpm were 2.14% (w/v) apSCT loading in citrate-phosphate buffer (50 mM, pH 6.0), recombinant hydrolytic enzymes (from Clostridium/Hungateiclostridium thermocellum) loading for endo-1,4-β-glucanase (CtCel8A) = 213.2 U/g, cellobiohydrolase (CtCBH5A) = 272.5 U/g and β-glucosidase (HtBg1) = 299.8 U/g for 49.2 h. Under optimized saccharification conditions, the total reducing sugar yield was 265 mg/g (glucose 214 mg/g) of apSCT. Fermentation of produced glucose by S. cerevisiae gave 0.19 g/g glucose of bioethanol.
More Related Videos
Related Concept Videos
Bioreactor Controls-III
Production of Alcohol
Production of Organic Acids

