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Long non-coding RNA KTN1-AS1 promotes progression in pancreatic cancer through regulating

Zhong-Bo Zhang1, Ning Liu1

  • 1Department of Pancreatic and Biliary Surgery, The First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.

Aging
|August 30, 2021
PubMed

Insights

Long non-coding RNA KTN1-AS1 promotes pancreatic cancer growth by inhibiting miR-23b-3p, which targets HMGB2. This discovery offers a potential diagnostic and therapeutic target for pancreatic cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Pancreatic cancer (PC) remains a significant health challenge with limited therapeutic options.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer development and progression.
  • Understanding the molecular mechanisms underlying PC pathogenesis is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the role of lncRNA KTN1-AS1 in pancreatic cancer progression.
  • To elucidate the regulatory axis involving KTN1-AS1, microRNA-23b-3p (miR-23b-3p), and high-mobility group box 2 (HMGB2) in PC cells.
  • To assess the diagnostic and prognostic value of KTN1-AS1 in pancreatic cancer.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to detect KTN1-AS1 expression.
  • Cell proliferation, invasion, and apoptosis assays (CCK-8, Transwell, flow cytometry) in transfected PC cells.
  • Dual-luciferase reporter assays and RNA immunoprecipitation (RIP) to confirm molecular interactions.

Main Results:

  • KTN1-AS1 was highly expressed in PC tissues and correlated with advanced clinicopathological features, including lymph node metastasis.
  • Downregulation of KTN1-AS1 inhibited PC cell proliferation and invasion while promoting apoptosis.
  • KTN1-AS1 directly targeted miR-23b-3p, and miR-23b-3p targeted HMGB2, suggesting a regulatory axis.
  • Overexpression of miR-23b-3p reversed the oncogenic effects of KTN1-AS1.

Conclusions:

  • KTN1-AS1 acts as an oncogenic lncRNA in pancreatic cancer by sponging miR-23b-3p and upregulating HMGB2.
  • KTN1-AS1 holds potential as a diagnostic biomarker and therapeutic target for pancreatic cancer.
  • The KTN1-AS1/miR-23b-3p/HMGB2 axis represents a novel regulatory pathway in pancreatic cancer progression.
Keywords:
HMGB2invasionlncRNA KTN1-AS1miR-23b-5ppancreatic cancerproliferation

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