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An alternative D. melanogaster 7SK snRNP.

Duy Nguyen¹, Nicolas Buisine², Olivier Fayol¹

¹Université Paris-Saclay, INSERM, CNRS, Interactions cellulaires et physiopathologie hépatique, Bât.440, 91405, Orsay, France.

BMC Molecular and Cell Biology

|August 31, 2021

Summary

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Researchers discovered a new 7SK small nuclear RNA (snRNA) in fruit flies. This novel RNA interacts with key protein partners, suggesting a dual role for 7SK snRNA in gene regulation.

Area of Science:

Molecular Biology
Genetics
RNA Biology

Background:

The 7SK small nuclear RNA (snRNA) is crucial for regulating P-TEFb, which controls RNA polymerase II elongation.
While sequence varies, 7SK RNA's structure and function are conserved across diverse species.

Purpose of the Study:

To identify and characterize a novel ncRNA in Drosophila melanogaster with 7SK RNA features.
To investigate the gene structure, expression, and biological function of this new RNA.

Main Methods:

Gene structure examination and RNA expression profiling during development.
In vivo interaction studies with P-TEFb complex partners (HEXIM, LARP7, Cyclin T).

Main Results:

A novel ncRNA, snRNA:7SK:94F, was identified in D. melanogaster, likely processed from a lncRNA.

Keywords:

7SK snRNA Drosophila Long non-coding RNA P-TEFb

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This alternative 7SK RNA interacts with HEXIM, LARP7, and Cyclin T, key components of the P-TEFb complex.

The novel RNA is expressed across various tissues in the fruit fly.

Conclusions:

Drosophila melanogaster possesses two distinct 7SK genes.
These genes likely cooperate in forming the 7SK snRNP complex, contributing to gene regulation.