Photochemiluminescent detection of antiradical activity. I. Assay of superoxide dismutase
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I N Popov1, G Lewin, R von Baehr
1Institute of Medical Immunology, Humboldt University, Berlin, GDR.
Biomedica Biochimica Acta
|January 1, 1987
Abstract:
The luminol sensitized photogeneration of superoxide radicals was combined with their luminol-dependent chemiluminescent detection. The inhibition of chemiluminescence by superoxide dismutase (SOD) versus enzyme concentration in reciprocals was strongly linear. The amount of SOD notable for 50% inhibition was between 115 and 500 ng for different enzyme preparations.