Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

53.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.5K
RNA Editing02:23

RNA Editing

9.3K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.3K
RNA Interference01:23

RNA Interference

26.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.8K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

683
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
683
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.9K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.9K
Experimental RNAi02:15

Experimental RNAi

6.5K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hawaiian geothermal fumaroles contain diverse and novel viruses.

Microbiology spectrum·2026
Same author

Hawaiian Geothermal Fumaroles Contain Diverse and Novel Viruses.

bioRxiv : the preprint server for biology·2026
Same author

END nucleases are antiphage defence systems targeting multiple phages with modified genomes.

Nature microbiology·2026
Same author

Ribosomal RNA cleavage by the previously unidentified RelS-RelI toxin-antitoxin system controls growth of Mycobacterium tuberculosis.

Nucleic acids research·2026
Same author

Expanding the atlas of bacterial immunity with biological language models.

Molecular cell·2026
Same author

Beyond oncogenesis: the unexplored benefits of viruses in cancer immunity.

Nature reviews. Cancer·2026

Related Experiment Video

Updated: Oct 22, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.1K

Compact RNA editors with small Cas13 proteins.

Soumya Kannan1,2,3,4,5, Han Altae-Tran1,2,3,4,5, Xin Jin1,2,3,4,5,6,7

  • 1Howard Hughes Medical Institute, Cambridge, MA, USA.

Nature Biotechnology
|August 31, 2021
PubMed
Summary

Researchers engineered compact CRISPR-Cas13bt RNA editors for mammalian transcript knockdown. These novel editors, packaged in adeno-associated virus, offer precise, temporary RNA modification for therapeutic applications.

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.6K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.6K

Related Experiment Videos

Last Updated: Oct 22, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.1K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.6K
Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

27.6K

Area of Science:

  • Molecular Biology
  • Gene Editing Technologies
  • RNA Therapeutics

Background:

  • CRISPR-Cas13 systems enable precise RNA editing for transient modifications.
  • Current RNA editing tools face challenges in delivery and efficiency.
  • Cas13b proteins offer a platform for RNA manipulation.

Purpose of the Study:

  • To identify and characterize novel, ultrasmall Cas13b variants for RNA editing.
  • To engineer compact CRISPR-based RNA editors (REPAIR and RESCUE) using Cas13bt.
  • To demonstrate the in vivo packaging and potential therapeutic application of these editors.

Main Methods:

  • Identification and characterization of ultrasmall Cas13b proteins (Cas13bt).
  • Engineering of REPAIR and RESCUE RNA editors by fusing Cas13bt with deaminase domains.
  • Packaging of engineered editors into adeno-associated virus (AAV) vectors.

Main Results:

  • Discovery of an ultrasmall Cas13bt family capable of mammalian transcript knockdown.
  • Successful engineering of compact REPAIR and RESCUE RNA editors utilizing Cas13bt.
  • Demonstration of packaging these novel RNA editors within a single AAV vector.

Conclusions:

  • Cas13bt provides a highly compact and efficient platform for RNA editing.
  • Engineered Cas13bt-based editors are suitable for AAV delivery, enabling therapeutic RNA modification.
  • These advancements expand the toolkit for precise and transient RNA-based interventions.